Thermo Fisher Scientific Phospho-c-Myc (Ser62) Polyclonal Antibody

Applications

Western Blot (WB)

• Tested Dilution: 1:500–1:1,000
• Publications: View 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

• Tested Dilution: 1:50–1:200
• Publications: View reference

Product Specifications

Product Specific Information

This antibody detects endogenous c-Myc protein at molecular weights of 49, 57, and 65 kDa.
Purity is >95% by SDS-PAGE.

Target Information

The c-Myc protein is a 62 kDa transcription factor encoded by the c-Myc gene on human chromosome 8q24. It is commonly activated in various tumor cells and plays a crucial role in cellular proliferation, differentiation, apoptosis, and cell cycle progression.

Phosphorylation at Thr58/Ser62 by glycogen synthase kinase 3, cyclin-dependent kinase, ERK2, and JNK is associated with cell proliferation and cycle regulation. c-Myc is essential for vasculogenesis and angiogenesis during tumor development.

The c-myc oncogene regulates normal cellular proliferation and differentiation, while deregulated expression induces apoptosis. Antibodies against c-myc epitopes recognize overexpressed proteins containing Myc epitope tags fused to either amino- or carboxy-termini.

Amplification of the c-Myc gene is found in various human tumors including lung, breast, and colon carcinomas. N-Myc amplification is associated with neuroblastomas. Translocation of the c-myc locus on chromosome 8 to immunoglobulin loci (14, 2, or 22) is observed in Burkitt’s lymphoma and other B-cell lymphoproliferative conditions.

Aberrant expression of c-myc occurs in tumors of multiple origins such as colorectal, gastric, hepatic, mammary, ovarian, pulmonary, and hematopoietic neoplasms.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.