Thermo Fisher Scientific Phospho-c-Myc (Ser373) Polyclonal Antibody
Phospho-c-Myc (Ser373) 폴리클로날 항체로 인간, 마우스, 랫트 시료에서 c-Myc 인산화 단백질 검출에 사용됩니다. Western blot, IHC(P), IP에 적합하며, 항원 친화 크로마토그래피로 정제되었습니다. 세포 증식 및 종양 연구용으로 적합합니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:1,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
| Immunoprecipitation (IP) | 1:50–1:200 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic phosphopeptide derived from human Myc around the phosphorylation site of Serine 373 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2553652 |
Product Specific Information
- Detects endogenous protein at molecular weights of 49, 57, and 65 kDa.
- Purity >95% by SDS-PAGE.
Target Information
The c-Myc protein is a 62 kDa transcription factor encoded by the c-Myc gene on human chromosome 8q24.
It is frequently activated in tumor cells and plays key roles in:
- Cellular proliferation, differentiation, apoptosis, and cell cycle progression
- Phosphorylation at Thr58/Ser62 by GSK3, CDK, ERK2, and JNK
- Vasculogenesis and angiogenesis during tumor development
Abnormal c-Myc expression is associated with multiple cancers including lung, breast, colon, and neuroblastoma.
Translocation of the c-Myc locus is characteristic of Burkitt’s lymphoma and other B-cell proliferative disorders.
Antibodies against c-Myc epitopes can detect overexpressed or tagged proteins containing Myc sequences.
For Research Use Only.
Not for use in diagnostic procedures or resale without authorization.
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