Thermo Fisher Scientific Cripto Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

2 µg/mL

-

Immunocytochemistry (ICC/IF)

1:1,000

-

Flow Cytometry (Flow)

1:100

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Product Specifications

Species Reactivity

Human, Mouse

Published species

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A synthetic peptide made to an N-terminal portion of the murine cripto protein sequence (between residues 1-50). if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:Cripto,uniProtId:P51865-1,ncbiNodeId:10090,antigenRange:50,antigenLength:171,antigenImageFileName:PA1-16534_Cripto_P51865-1_Rabbit.svg,antigenImageFileNamePDP:PA1-16534_Cripto_P51865-1_Rabbit_PDP.jpeg,sortOrder:2}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1.0 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS

Contains

0.02% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_568503

Product Specific Information

In Western blot a band is seen at ~18 kDa representing the core form of Cripto1, and at ~36 kDa reporesenting the glycosylated/ post-translationally modified form.

Suggested positive control: MDA-MB231 (human breast cancer) lysates.

Target Information

Cripto (TDGF1) is a GPI-anchored cell membrane proetin involved in Nodal signaling. Cell associated TDGF1 acts as a a Nodeal coreceptor in cis. Shedding of TDGF1 by TMEM8A modulated Nodal signaling by allowing soluble TDGF1 to act as a Nodal coreceptor on other cells. TDGF1 is expressed both in ES cells and during the early phases of embryo development, while in adults it is reactivated in a wide range of epithelial cancers.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.