Thermo Fisher Scientific ERK2 Internal Polyclonal Antibody, DyLight 680
DyLight™ 680 형광 표지된 ERK2 내부 폴리클로날 항체로, 인간·마우스·랫트 반응성을 가집니다. Western blot 및 ELISA에 최적화되어 있으며, ERK1과 비반응성입니다. MAPK1(ERK2) 연구용으로 사용되며 장기 보관 시 -20°C에서 안정적입니다.
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Thermo Fisher Scientific ERK2 Internal Polyclonal Antibody, DyLight™ 680
Applications and Tested Dilution
- Western Blot (WB): 1 µg/mL
- ELISA: 1:40,000
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host/Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide corresponding to amino acid residues near an internal region of ERK2 conjugated to KLH |
| Conjugate | DyLight™ 680 |
| Excitation/Emission Max | 676/705 nm |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | 0.02M potassium phosphate, pH 7.2, with 0.15M NaCl, 10 mg/mL BSA |
| Contains | 0.01% sodium azide |
| Storage Conditions | Store at 4°C short term; for long-term, store at -20°C, avoiding freeze/thaw cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Store vial at 4°C prior to restoration.
- For extended storage, aliquot and freeze at -20°C or below.
- Avoid freeze/thaw cycles.
- Centrifuge if not clear after standing at room temperature.
- Stable for several weeks at 4°C as undiluted liquid.
- Dilute only prior to immediate use.
This DyLight™ 680 conjugated affinity-purified antibody is directed against human ERK2 protein.
Cross-reactivity is expected with human, mouse, and rat based on sequence identity of the peptide immunogen.
This antibody does not react with the ERK1 isoform.
Target Information
MAPK1 (ERK2) is a serine/threonine kinase that acts as an important activator of p90 RSK, MSK, ELK1, and Stat3, and is involved in the MAPK pathway.
MAP kinases (ERKs) integrate multiple biochemical signals and regulate cellular processes such as proliferation, differentiation, transcription, and development.
Activation requires phosphorylation by upstream kinases, after which ERK2 translocates to the nucleus to phosphorylate nuclear targets.
For Research Use Only. Not for use in diagnostic procedures or resale without authorization.
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