Thermo Fisher Scientific MYC Epitope Tag Polyclonal Antibody, DyLight 800
MYC epitope tag 인식용 DyLight 800 결합 토끼 폴리클로날 항체. Western blot, ICC/IF, ELISA에 적합. 높은 특이성과 민감도를 제공하며, MYC 태그 단백질 검출에 유용. 장기 보관 시 -20°C에서 안정적.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:10,000–1:25,000 |
| Immunocytochemistry (ICC/IF) | >1:5,000 |
| ELISA | >1:20,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Tag |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | This antibody was purified from whole rabbit serum prepared by repeated immunizations with MYC epitope tag peptide (E-Q-K-L-I-S-E-E-D-L) conjugated to KLH using maleimide. The sequence corresponds to amino acids 410–419 of human c-Myc. |
| Conjugate | DyLight™ 800 |
| Excitation / Emission Max | 789 / 794 nm |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage buffer | 0.02M potassium phosphate (pH 7.2), 0.15M NaCl, 10 mg/mL BSA |
| Contains | 0.01% sodium azide |
| Storage conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
Store vial at 4°C prior to restoration. For extended storage, aliquot contents and freeze at -20°C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use.
This affinity-purified antibody is directed against the MYC epitope tag and is useful for determining its presence in overexpressed proteins in various assays. The antibody recognizes the MYC epitope tag (Glu-Gln-Lys-Leu-Ile-Ser-Glu-Glu-Asp-Leu) fused to either the amino- or carboxy-termini of targeted proteins in transfected or transformed cells.
Target Information
Myc is a proto-oncogene encoding a nuclear phosphoprotein involved in cell cycle progression, apoptosis, and cellular transformation. The encoded protein forms a heterodimer with transcription factor MAX, binding to the E-box DNA consensus sequence to regulate gene transcription. Amplification of this gene is frequently observed in numerous human cancers. Translocations involving this gene are associated with Burkitt lymphoma and multiple myeloma. Translation may initiate from both an upstream non-AUG (CUG) and a downstream AUG start site, producing two isoforms with distinct N-termini.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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