Thermo Fisher Scientific IL-33R (ST2) Monoclonal Antibody (RMST2-2), eFluor 450, eBioscience

Thermo Fisher Scientific IL-33R (ST2) Monoclonal Antibody (RMST2-2), eFluor 450, eBioscience

Applications

• Immunohistochemistry (IHC)

  • Not tested
  • View 1 publication

• Flow Cytometry (Flow)

  • Tested dilution: 0.5 µg/test
  • View 5 publications

Product Specifications

Available Formats

• Alexa Fluor 488
• APC
• APC-eFluor 780
• Biotin
• Brilliant UV 395 / 615 / 737
• Brilliant Violet 421
• PE / PE-Cyanine7
• PerCP-eFluor 710
• Super Bright 436 / 600 / 645
• Request custom conjugation

Product Specific Information

The RMST2-2 monoclonal antibody reacts with ST2 (IL-33 receptor, IL-33R), a member of the IL-1 receptor family. The membrane-bound form consists of three extracellular immunoglobulin domains and an intracellular toll-interleukin-1 receptor domain. ST2 forms a dimer with IL-1R accessory protein in a ligand-dependent manner, and IL-33/ST2 interaction induces TH2 cytokine production.

ST2 also has a soluble isoform lacking transmembrane and intracellular domains, functioning as a decoy receptor blocking IL-33/ST2 interaction. ST2 is expressed by TH2 lymphocytes, mast cells, eosinophils, basophils, innate lymphocytes, smooth muscle cells, and endothelial cells, involved in host defense, allergy, and inflammation. The IL-33/ST2 interaction is known to be atheroprotective.

Applications Reported: Flow cytometric analysis.
Applications Tested: Flow cytometric analysis of mouse bone marrow-derived mast cells at ≤0.5 µg/test (100 µL final volume).
Cell number range: 10⁵–10⁸ cells/test. Antibody titration recommended for optimal performance.

Fluorochrome Information:
eFluor 450 is an alternative to Pacific Blue, emitting at 446 nm and excited at 405 nm (violet laser). Ensure instrument compatibility.
Excitation: 405 nm | Emission: 445 nm | Laser: Violet

Target Information

IL-33R (ST2) is a receptor for IL-33, belonging to the IL-1 receptor family. Its extracellular domain includes 3 Ig-like domains that interact with IL-1RAcP and IL-33. IL-33 binding activates NF-κB and MAPK pathways, inducing cytokines such as IL-5, IL-6, IL-8, and IL-13.

Two isoforms exist via alternative splicing:

• ST2L (membrane-bound)
• ST2S (soluble, decoy receptor)

IL-33R is primarily expressed by mast cells, basophils, eosinophils, and in mouse, ILC2 and Th2 cells. IL-33R signaling is implicated in allergies, arthritis, atherosclerosis, and sepsis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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