Thermo Fisher Scientific H4ac pan-acetyl (K5,K8,K12,K16) Polyclonal Antibody
히스톤 H4의 4개 리신 잔기(K5, K8, K12, K16) 아세틸화 형태를 인식하는 폴리클로날 항체. WB, ICC/IF, ELISA, IP, ChIP 등 다양한 응용에 적합. 인간, 마우스, 랫트 반응성. 연구용 전용, 장기 보관 시 -20°C 권장.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:800 | View 1 publication |
| Immunohistochemistry (IHC) | - | View 1 publication |
| Immunohistochemistry (PFA fixed) (IHC (PFA)) | - | View 1 publication |
| Immunocytochemistry (ICC/IF) | 1:200 | View publication |
| ELISA | 1:400 | View publication |
| Immunoprecipitation (IP) | Assay-dependent | View publication |
| ChIP assay (ChIP) | Assay-dependent | View 2 publications |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | All, Human, Mouse, Rat |
| Published Species | Human, Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | KLH-conjugated peptide acetylated at lysines 5, 8, 12, 16: NSGRGAcKGGAcKGLGAcKGGAAcKRHYCC |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | Not Determined |
| Storage Buffer | Whole serum |
| Contains | <0.1% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2116718 |
Product Specific Information
Rabbit anti-Histone H4 (Acetylated) antibody was raised against the tetra-acetylated form of histone H4 and recognizes acetylated isoforms of histone H4.
Target Information
Histones are basic nuclear proteins responsible for the nucleosome structure of chromosomal fiber in eukaryotes. Two molecules each of H2A, H2B, H3, and H4 form an octamer around which ~146 bp of DNA is wrapped in repeating units called nucleosomes.
The linker histone H1 interacts with linker DNA between nucleosomes and aids chromatin compaction into higher-order structures.
This gene is intronless and encodes a member of the histone H4 family. Transcripts lack polyA tails but contain a palindromic termination element and are located in the large histone gene cluster on chromosome 6.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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