Thermo Fisher Scientific CD326 (EpCAM) Monoclonal Antibody (G8.8), Super Bright 645, eBioscience
마우스 EpCAM(CD326)을 인식하는 G8.8 클론 단일클론 항체로, Super Bright 645 형광체로 결합됨. 유세포 분석(Flow Cytometry)에 최적화되어 있으며, 405 nm 레이저에서 여기되어 645 nm 방출. 암세포 및 상피세포 표지용 연구에 적합.
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Thermo Fisher Scientific CD326 (EpCAM) Monoclonal Antibody (G8.8), Super Bright 645, eBioscience
Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunohistochemistry (IHC) | - | View 2 publications |
| Immunohistochemistry (Frozen) (IHC (F)) | - | View 1 publication |
| Immunocytochemistry (ICC/IF) | - | View 5 publications |
| Flow Cytometry (Flow) | 0.25 µg/test | View 26 publications |
Product Specifications
| Specification | Detail |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Not Applicable |
| Host / Isotype | Rat / IgG2a, kappa |
| Recommended Isotype Control | Rat IgG2a kappa Isotype Control (eBR2a), Super Bright 645, eBioscience |
| Class | Monoclonal |
| Type | Antibody |
| Clone | G8.8 |
| Conjugate | Super Bright™ 645 |
| Excitation / Emission Max | 414 / 645 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2688103 |
Available Formats
- Unconjugated
- Alexa Fluor 700
- APC
- APC-eFluor 780
- Biotin
- Brilliant Violet 711
- Brilliant Violet 786
- eFluor 450
- FITC
- PE
- PE-Cyanine5
- PE-Cyanine7
- PE-eFluor 610
- PerCP-eFluor 710
- Super Bright 436
- Super Bright 600
- Request custom conjugation
Product Specific Information
The G8.8 monoclonal antibody reacts with the 40 kDa mouse EpCAM (epithelial cellular adhesion molecule), also known as EGP40, 17-1A antigen, TACSTD1, and CD326. The immunogen used was the TE-71 thymic epithelial cell line. CD326 is expressed on most epithelial cells and is considered a pan-carcinoma antigen. It mediates calcium-independent, homophilic cell-cell adhesion and may act as a growth factor receptor.
Applications Reported: Suitable for flow cytometric analysis.
Applications Tested: Tested on TE-71 cell line, recommended ≤0.25 µg/test (100 µL volume, 10⁵–10⁸ cells/test).
Super Bright 645 is a tandem dye excited by 405 nm violet laser and emits at 645 nm. Recommended filter: 660/20 bandpass. Ensure instrument compatibility.
When using multiple Super Bright dye-conjugated antibodies, use Super Bright Complete Staining Buffer (Product # SB-4401) to minimize non-specific interactions.
Light Sensitivity: Protect from light to prevent photo-induced oxidation.
Fixation: Samples may be stored in IC Fixation Buffer (Product # 00-8222) or 1-step Fix/Lyse Solution (Product # 00-5333) for up to 3 days at 4°C in the dark with minimal signal loss.
Excitation: 405 nm
Emission: 645 nm
Laser: Violet Laser
Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.
Target Information
Ep-CAM (epithelial adhesion molecule, ESA) is a transmembrane glycoprotein (~40 kDa) expressed in epithelial tissues. Functions as a calcium-independent homotypic adhesion molecule and influences cell cycle and proliferation by inducing c-myc and cyclin A/E. Overexpression correlates with enhanced epithelial proliferation and is a marker for epithelial-derived tumors. Expressed on baso-lateral surfaces of most simple epithelia and many carcinoma types. Also distinguishes adenocarcinomas from pleural mesotheliomas.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without authorization.
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