Thermo Fisher Scientific CD244 Monoclonal Antibody (eBioC1.7 (C1.7)), Super Bright 645, eBioscience

Thermo Fisher Scientific CD244 Monoclonal Antibody (eBioC1.7 (C1.7)), Super Bright 645, eBioscience

Applications

• Flow Cytometry (Flow)

Tested Dilution: 5 µL (1.0 µg)/test

Product Specifications

Additional Formats

• Biotin
• Functional Grade
• NovaFluor Blue 660-120S
• NovaFluor Red 685
• NovaFluor Red 700
• NovaFluor Red 755
• NovaFluor Yellow 610
• NovaFluor Yellow 660
• NovaFluor Yellow 700
• PE
• PE-Cyanine5.5
• Super Bright 436
• Super Bright 600
• Request custom conjugation available

Product Specific Information

The eBioC1.7 monoclonal antibody reacts with human CD244 (2B4, p38), a 38 kDa protein expressed on NK cells, CD8+ T cells, γδ T cells, monocytes, basophils, and eosinophils. Binding of CD48 to CD244 enhances NK cell cytotoxicity and IFN-γ secretion. The C1.7 clone binds to the same residue as CD48, inducing NK cell activation via tyrosine phosphorylation and SAP recruitment.

Applications Reported: Suitable for flow cytometric analysis.
Applications Tested: Pre-diluted and tested on normal human peripheral blood cells. Use at 5 µL (1 µg/mL) per test (100 µL final volume, 10⁵–10⁸ cells/test).

Super Bright 645 is a tandem dye excited by a 405 nm violet laser and emits at 645 nm. Recommended filter: 660/20 bandpass. Ensure instrument compatibility.

Use Super Bright Complete Staining Buffer (Product # SB-4401) when combining multiple Super Bright dyes to minimize non-specific polymer interactions.

Light sensitivity: Protect from light to avoid photo-induced oxidation.
Fixation: Samples may be stored in IC Fixation Buffer (Product # 00-8222) or 1-step Fix/Lyse Solution (Product # 00-5333) for up to 3 days at 4°C in the dark with minimal brightness loss.

Excitation: 405 nm
Emission: 645 nm
Laser: Violet Laser

Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.

Target Information

CD244 (2B4, SLAMF4) is a surface receptor on NK cells and some T cells, mediating non-MHC restricted killing. Interaction with SLAMF2 activates both SLAMF4- and SLAMF2-expressing cells. Reduced SLAMF4 levels are observed in systemic lupus erythematosus, suggesting a role in autoimmune pathology. CD244 dysfunction is linked to rheumatoid arthritis and lymphoproliferative syndrome. Alternative splicing produces multiple isoforms.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without authorization.

제품 이미지