Thermo Fisher Scientific IgM Monoclonal Antibody (II/41), Super Bright 645, eBioscience
마우스 IgM 무거운 사슬을 인식하는 II/41 단클론 항체로, Super Bright 645 형광체가 결합된 제품입니다. Flow cytometry에 최적화되어 있으며, 높은 감도와 낮은 비특이적 결합을 제공합니다. 405 nm 레이저로 여기되어 645 nm에서 방출됩니다.
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Thermo Fisher Scientific IgM Monoclonal Antibody (II/41), Super Bright 645, eBioscience™
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunohistochemistry (IHC) | - | View 1 publication |
| Flow Cytometry (Flow) | 1.0 µg/test | View 5 publications |
| ELISA | - | View 3 publications |
Product Specifications
| Specification | Detail |
|---|---|
| Published Species | Not Applicable |
| Host / Isotype | Rat / IgG2a, kappa |
| Recommended Isotype Control | Rat IgG2a kappa Isotype Control (eBR2a), Super Bright™ 645, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | II/41 |
| Conjugate | Super Bright™ 645 |
| Excitation / Emission Max | 414 / 645 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Wet ice |
| RRID | AB_2723277 |
Available Formats
- Unconjugated
- APC
- APC-eFluor 780
- Biotin
- Brilliant Violet 605
- Brilliant Violet 711
- eFluor 450
- eFluor 570
- eFluor 660
- FITC
- NovaFluor Red 710
- NovaFluor Yellow 610
- PE
- PE-Cyanine5
- PE-Cyanine7
- PE-eFluor 610
- PerCP-eFluor 710
- Super Bright 436
- Super Bright 600
- Super Bright 780
- Request custom conjugation
Product Specific Information
The II/41 monoclonal antibody reacts with the mu heavy chain of mouse IgM. It does not react with other classes of mouse immunoglobulin including IgD, IgG or IgA. IgM is expressed intracellularly during early stages of B lymphopoiesis and on the surface of mature B cells.
- Applications Reported: Flow cytometric analysis.
- Applications Tested: Tested by flow cytometry on mouse bone marrow cells at ≤1 µg/test (100 µL volume). Optimal titration recommended.
- Fluorochrome: Super Bright 645, excited at 405 nm, emits at 645 nm. Use 660/20 bandpass filter.
- Light Sensitivity: Protect from light due to photo-induced oxidation.
- Fixation: Samples can be stored in IC Fixation Buffer (Product #00-8222) or 1-step Fix/Lyse Solution (Product #00-5333) for up to 3 days at 4°C in the dark.
- Recommended Buffer: Use Super Bright Complete Staining Buffer (Product #SB-4401) when using multiple Super Bright conjugates.
Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.
Target Information
Mouse IgM is an immunoglobulin M antibody produced by B cells in response to antigens. It is the first antibody produced during an immune response and plays a key role in complement activation and antigen agglutination. Composed of five heavy and five light chains, IgM is crucial in innate immunity. It is widely used in ELISA, Western blotting, and immunohistochemistry research applications.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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