Thermo Fisher Scientific NOTCH 2 Polyclonal Antibody
Human NOTCH2 단백질을 인식하는 Rabbit Polyclonal Antibody로, WB, IHC, ELISA, IP 등에 사용 가능. NICD N-terminal 부위에 대한 합성 펩타이드로 면역화됨. 높은 특이성과 반응성, 연구용으로 적합. -20°C 보관 권장.
- 카탈로그번호
- 100-401-408x (2개 옵션)
- 판매단위
- pk
카탈로그
2개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific NOTCH 2 Polyclonal Antibody
Thermo Fisher Scientific NOTCH 2 Polyclonal Antibody
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:400–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:500 |
| ELISA | 1:20,000–1:100,000 |
| Immunoprecipitation (IP) | 1:400–1:2,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Whole rabbit serum prepared by repeated immunizations with a synthetic peptide corresponding to amino acid residues of human NOTCH2 near the N-terminal sequence of the cleaved N intracellular domain (NICD). |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 80 mg/mL |
| Storage Buffer | 0.02M potassium phosphate/whole serum, pH 7.2, with 0.15M NaCl |
| Contains | 0.01% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Store vial at -20°C prior to opening.
- Aliquot contents and freeze at -20°C or below for extended storage.
- Avoid cycles of freezing and thawing.
- Centrifuge product if not completely clear after standing at room temperature.
- Stable for several weeks at 4°C as an undiluted liquid.
- Dilute only prior to immediate use.
This antiserum is directed against human NOTCH2. The immunogen epitope is exposed only after gamma secretase cleavage and not accessible in the uncleaved form.
Based on sequence analysis, this antibody is expected to also react with rat and mouse NOTCH2, with only one conservative amino acid difference.
Target Information
Neurogenic locus notch homolog protein 2 (NOTCH2) is a highly conserved protein in the Notch signaling pathway.
It is a type 1 transmembrane protein with:
- Extracellular domain containing multiple EGF-like repeats
- Intracellular domain composed of several distinct domain types
NOTCH2 acts as a receptor for membrane-bound ligands such as Jagged1, Jagged2, and Delta1, regulating cell fate determination.
Defects in NOTCH2 are associated with Alagille syndrome type 2 (ALGS2), an autosomal dominant multisystem disorder characterized by hepatic bile duct paucity and cholestasis, along with cardiac, skeletal, and ophthalmologic manifestations.
Other features include characteristic facial morphology and occasional renal or vascular involvement.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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(이미지 파일명: 100-401-408_NOTCH2_Q04721-1_Rabbit.svg, 100-401-408_NOTCH2_Q04721-1_Rabbit_PDP.jpeg)
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