Thermo Fisher Scientific Chloramphenicol-Acetyl Transferase Polyclonal Antibody
Chloramphenicol-Acetyl Transferase 단백질을 인식하는 토끼 폴리클로날 항체로, WB, IHC, ELISA, IP 등에 사용 가능. 비결합형, 동결건조 형태로 제공되며 세균 시료에 반응. 연구용으로 CAT 단백질 검출 및 발현 분석에 적합.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (IHC) | Assay-dependent |
| ELISA | 1:5,000–1:25,000 |
| Immunoprecipitation (IP) | 1:500–1:2,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Bacteria |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | This antiserum was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near amino acids 30–60 of bacterial chloramphenicol acetyl transferase (CAT). |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 85 mg/mL |
| Storage Buffer | 0.02 M potassium phosphate / whole serum, pH 7.2, with 0.15 M NaCl |
| Contains | 0.01% sodium azide |
| Storage Conditions | Store at 4°C short term. For long-term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
Store vial at 4°C prior to restoration. For extended storage, aliquot contents and freeze at -20°C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use.
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-rabbit serum, purified and partially purified chloramphenicol acetyl transferase (CAT). Cross-reactivity against CAT from other tissues and species may occur but has not been specifically determined.
Target Information
Chloramphenicol-Acetyl Transferase (CAT) is commonly used as a tag in various systems and transcription assays. The enzyme acts as an effector of chloramphenicol resistance in bacteria.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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