Thermo Fisher Scientific Phospho-Myosin (Ser19, Ser20) Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000-1:5,000

-

Immunohistochemistry (IHC)

2.5 µg/mL

-

ELISA (ELISA)

1:5,000-1:20,000

-

Immunoprecipitation (IP)

1:1,000-1:5,000

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Human Myosin Light Chain phospho peptide corresponding to a region near the amino terminus of the human smooth/non-muscle form of myosin regulatory light chain conjugated to Keyhole Limpet Hemocyanin (KLH). if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:MYL9/MYL12,uniProtId:P24844-1,ncbiNodeId:9606,antigenRange:1,antigenLength:172,antigenImageFileName:100-401-416_MYL9MYL12_P24844-1_Rabbit.svg,antigenImageFileNamePDP:100-401-416_MYL9MYL12_P24844-1_Rabbit_PDP.jpeg,sortOrder:1},{targetFamily:MYL9/MYL12,uniProtId:O14950-1,ncbiNodeId:9606,antigenRange:1,antigenLength:172,antigenImageFileName:100-401-416_MYL9MYL12_O14950-1_Rabbit.svg,antigenImageFileNamePDP:100-401-416_MYL9MYL12_O14950-1_Rabbit_PDP.jpeg,sortOrder:2},{targetFamily:MYL9/MYL12,uniProtId:P19105-1,ncbiNodeId:9606,antigenRange:1,antigenLength:171,antigenImageFileName:100-401-416_MYL9MYL12_P19105-1_Rabbit.svg,antigenImageFileNamePDP:100-401-416_MYL9MYL12_P19105-1_Rabbit_PDP.jpeg,sortOrder:3}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

70 mg/mL

Storage buffer

0.02M potassium phosphate/whole serum, pH 7.2, with 0.15M NaCl

Contains

0.01% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

Product Specific Information

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Anti-Myosin pS19/pS20 antibody is directed against the regulatory light chain of smooth and non-muscle myosin. Reactivity with non-phosphorylated myosin light chain is less than 1% by ELISA. Cross reactivity is expected with myosin light chain from human and mouse. Reactivity with the protein from other species has not been determined. However, the sequence of the immunogen is nearly identical in mammalian and avian species. BLAST search analysis was used to determine that the smooth and non-muscle forms of myosin regulatory light chain have identical sequences. Cross reactivity is expected.

Target Information

Myosin regulatory light polypeptide 9 is a highly conserved, ubiquitously expressed protein that in humans is encoded by the MYL9 gene. Myosin, a structural component of muscle, are hexameric proteins consisting of two heavy chains and four light chains, including a single pair of nonphosphorylatable light chain subunits and another pair of phosphorylatable light chain subunits.The protein encoded by this gene is a myosin light chain that may regulate muscle contraction by modulating the ATPase activity of myosin heads. Myosin light chain (Myl) 9 and Myl12 are new functional ligands for CD69. Blockade of CD69-Myl9/12 interactio ameliorates allergic airway inflammation in ovalbumin-induced and house dust mite-induced mouse models of asthma.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.