Thermo Fisher Scientific Glucocorticoid Receptor Monoclonal Antibody (BuGR2)

Applications

Tested Dilution

Publications

Western Blot (WB)

5 µg/mL

View 72 publications 72 publications

Immunohistochemistry (IHC)

-

View 9 publications 9 publications

Immunohistochemistry (Paraffin) (IHC (P))

5 µg/mL

-

Immunohistochemistry (PFA fixed) (IHC (PFA))

1:500

-

Immunohistochemistry (Frozen) (IHC (F))

-

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

Assay-dependent

View 13 publications 13 publications

Flow Cytometry (Flow)

1-2 µg/test

View 4 publications 4 publications

ELISA (ELISA)

-

View 2 publications 2 publications

Immunoprecipitation (IP)

Assay-dependent

View 22 publications 22 publications

ChIP assay (ChIP)

-

View 20 publications 20 publications

Gel Shift (GS)

Assay-dependent

View 5 publications 5 publications

Miscellaneous PubMed (Misc)

-

View 1 publication 1 publication

Product Specifications

Species Reactivity

Guinea pig, Human, Mouse, Sheep, Rabbit, Rat, Yeast

Published species

Human, Mouse, Non-human primate, Rabbit, Rat, Sheep, Xenopus, Yeast

Host/Isotype

Mouse / IgG2a

Class

Monoclonal

Type

Antibody

Clone

BuGR2

Immunogen

Partially purified rat GR. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:Glucocorticoid Receptor (Nr3c1),uniProtId:P06536-1,ncbiNodeId:10114,antigenRange:1-795,antigenLength:795,antigenImageFileName:MA1-510_Glucocorticoid_Receptor_Nr3c1_P06536-1_House_mouse.svg,antigenImageFileNamePDP:MA1-510_Glucocorticoid_Receptor_Nr3c1_P06536-1_House_mouse_PDP.jpeg,sortOrder:3}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.2

Contains

0.05% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_325427

Product Specific Information

MA1-510 detects glucocorticoid receptor (GR) from human, mouse, rat, guinea pig, rabbit, sheep and yeast samples. This antibody does not react with primate, avian or amphibian GR.

MA1-510 has been successfully used in Western blot, immunofluorescence, immunocytochemistry, flow cytometry, immunohistochemistry, immunoprecipitation, and gel shift procedures. By Western blot, this antibody detects a 97 kDa protein representing GR in L929 cell extract. Immunocytochemical staining of GR in L929 cells with MA1-510 results in staining of both the cytoplasm and nucleus, even in the presence of hormone. Using enzymatic digestion analysis, MA1-510 reacts with the undigested 97 kDa GR, a 17 kDa DNA-binding trypsin fragment, and a 45 kDa steroid- and DNA-binding chymotrypsin fragment.

The MA1-510 immunogen is partially purified rat GR.

Reconstitute with 100 µL distilled water.

Target Information

Glucocorticoid Receptor (NR3C1) is a receptor for glucocorticoids that can act as both a transcription factor and as a regulator of other transcription factors. Glucocorticoid Receptor can also be found in heteromeric cytoplasmic complexes along with heat shock factors and immunophilins. The protein is typically found in the cytoplasm until it binds a ligand, which induces transport into the nucleus. Glucocorticoid Receptor is expressed in the heart, detected in left and right atria, left and right ventricles, aorta, apex, intraventricular septum, and atrioventricular node as well as whole adult and fetal heart. Alternate splicing, the use of at least three different promoters, and alternate translation initiation sites result in several transcript variants encoding the same protein or different isoforms, but the full-length nature of some variants has not been determined. Mutations in the Glucocorticoid Receptor gene are a cause of glucocorticoid resistance, or cortisol, resistance. Studies have shown that glucocorticoid receptor (GR) must be associated with a complex of chaperone proteins for ligand activation. GR binds to known steroids such as dexamethasone with nanomolar affinity

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.