Thermo Fisher Scientific Glucocorticoid Receptor Monoclonal Antibody (BuGR2)

Applications and Tested Dilutions

Product Specifications

Product Specific Information

MA1-510 detects glucocorticoid receptor (GR) from human, mouse, rat, guinea pig, rabbit, sheep, and yeast samples. This antibody does not react with primate, avian, or amphibian GR.

MA1-510 has been successfully used in Western blot, immunofluorescence, immunocytochemistry, flow cytometry, immunohistochemistry, immunoprecipitation, and gel shift assays.
By Western blot, it detects a 97 kDa protein representing GR in L929 cell extract.
Immunocytochemical staining of GR in L929 cells shows both cytoplasmic and nuclear localization, even in the presence of hormone.
Enzymatic digestion analysis shows reactivity with the undigested 97 kDa GR, a 17 kDa DNA-binding trypsin fragment, and a 45 kDa steroid- and DNA-binding chymotrypsin fragment.

The immunogen is partially purified rat GR.
Reconstitute with 100 µL distilled water.

Target Information

Glucocorticoid Receptor (NR3C1) is a receptor for glucocorticoids functioning as both a transcription factor and regulator of other transcription factors.
It forms heteromeric cytoplasmic complexes with heat shock factors and immunophilins, remaining cytoplasmic until ligand binding induces nuclear transport.
GR is expressed in various heart tissues and other organs.
Alternative splicing and promoter usage generate multiple transcript variants encoding identical or related isoforms.
Mutations in NR3C1 can cause glucocorticoid resistance.
GR binds steroids such as dexamethasone with nanomolar affinity and requires chaperone protein complexes for ligand activation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.