Thermo Fisher Scientific AHR Monoclonal Antibody (RPT1)

Applications

Product Specifications

Product Specific Information

MA1-514 detects the aryl hydrocarbon receptor (AhR) from human, mouse, and rat cells.
It has been successfully used in Western blotting, immunoprecipitation, immunofluorescence, and immunohistochemistry procedures.
By Western blot, this antibody detects an ~96 kDa protein representing AhR, with some minor non-specific bands observed in certain samples.
The immunogen corresponds to residues 12–31 from the human AhR protein, with amino acids 18–21 omitted.

Target Information

AHR (Ah Receptor) belongs to a family of proteins including its dimerization partner ARNT (HIF-1 Beta) and the Drosophila proteins PER and SIM.
AHR contains an N-terminal sequence of approximately 200 amino acids termed the PAS domain.
It binds to a specific DNA enhancer sequence and initiates transcription of the mRNA for the cytochrome P-450 (CYPIA1) gene.
AHR encodes a ligand-activated transcription factor involved in the regulation of biological responses to planar aromatic hydrocarbons.
It regulates xenobiotic-metabolizing enzymes such as cytochrome P450, with ligands including aromatic hydrocarbons and halogenated compounds such as TCDD.
In non-ligand activated cells, AHR is found complexed with HSP90 in the cytoplasm. Upon agonist binding, AHR translocates to the nucleus, dissociates from HSP90, and forms a heterodimer with ARNT.
Diseases associated with AHR include eosinophilic fasciitis and seborrheic dermatitis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.