Takara pRI 909 and pRI 910 binary vectors

The Ri pRI 909 and pRI 910 binary vectors are intended for Agrobacterium_-mediated plant transformation and contain a region of T-DNA for _Arabidopsis, tomato, tobacco, and rice transformation. The origin of the T-DNA is the vir region-lacking Rhizobium (Agrobacterium) rhizogenes Ri plasmid. pRI 909 and pRI 910 are also shuttle vectors and replicate autonomously in E. coli and Rhizobium (Agrobacterium).

The Ri pRI 909 and pRI 910 binary vectors are intended for Agrobacterium_-mediated plant transformation and contain a region of T-DNA for _Arabidopsis, tomato, tobacco, and rice transformation. The origin of the T-DNA is the vir region-lacking Rhizobium (Agrobacterium) rhizogenes Ri plasmid. pRI 909 and pRI 910 are also shuttle vectors and replicate autonomously in E. coli and Rhizobium (Agrobacterium).

In E. coli, these vectors are capable of achieving a high copy number because they contain the ColE1 replication origin. The ColE1 ori, as well as the mutant-type Ri plasmid replication of origin (Ri-ori), are each stably maintained in Rhizobium (Agrobacterium). These vectors also contain the kanamycin-resistance gene NPTIII for selection in E. coli and Rhizobium (Agrobacterium), and a mutant-type kanamycin-resistance gene NPTII for selection in plants.

The pRI 909 and pRI 910 vectors are capable of plant transformation in combination with Rhizobium (Agrobacterium) through a binary vector system. Stable plant chromosome target gene integration is possible with pRI 909 and pRI 910 because their cloning sites are located in close proximity to the T-DNA Right Border (RB) and not the NPTII plant selection marker. As a result, the target gene is not deleted.

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Overview

• Easy and stable plant chromosome target gene integration
• Improved _agrobacterium_-mediated plant transformation
• Applicable to various plants such as Arabidopsis, tomato, tobacco, and rice

More Information

Applications

• Rhizobium (Agrobacterium)-mediated plant transformation
• Arabidopsis, tomato, tobacco, rice transformation

Storage

–20°C

Properties

• Preparation: column purified
• Size: 9,168 bp

Form

10 mM, Tris-HCl (pH 8.0), 1 mM EDTA

Product citations

Nishiguchi, R., Takanami, M., and Oka, A. Characterization and sequence determination of the replicator region in the hairy-root-inducing plasmid pRiA 4b. Mol. Gen. Genet. 206, 1–8 (1987).

Ohba, T., Yoshioka, Y., Machida, C., and Machida, Y. DNA rearrangement associated with the integration of T-DNA in tobacco: an example for multiple duplications of DNA around the integration target. The Plant J. 7, 157–164 (1995).

Ooms, G. et al. Octopine Ti-plasmid deletion mutants of Agrobacterium tumefaciens with emphasis on the right side of the T-region Plasmid 7, 15–29 (1982).

Additional product information

Please see the product`s Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.