Thermo Fisher Scientific CD223 (LAG-3) Monoclonal Antibody (3DS223H), PE-Cyanine7, eBioscience

Thermo Fisher Scientific CD223 (LAG-3) Monoclonal Antibody (3DS223H), PE-Cyanine7, eBioscience

Applications and Tested Dilution

• Application: Flow Cytometry
• Tested Dilution: 5 µL (0.06 µg)/test
• Publications: View 7 publications

Product Specifications

Available Formats

• Alexa Fluor 647
• Alexa Fluor 660
• Alexa Fluor 700
• APC
• APC-eFluor 780
• Brilliant UV Series (395, 563, 661, 737, 805)
• Brilliant Violet Series (421, 480, 605, 650, 711, 786)
• eFluor 450, eFluor 506
• FITC
• NovaFluor Series (Blue, Red, Yellow variants)
• PE, PE-Cyanine5, PE-eFluor 610
• PerCP-eFluor 710
• Super Bright Series (436, 600, 645, 702, 780)
• Request custom conjugation

Product Specific Information

This 3DS223H monoclonal antibody recognizes human CD223 (Lymphocyte Activation Gene 3, LAG-3), a 70-kDa surface glycoprotein of the Ig superfamily with homology to CD4. LAG-3 binds MHC class II with higher affinity than CD4 and negatively regulates T cell activation and proliferation. It is expressed on activated T cells, regulatory T cells, and NK cells, with CD8+ T cells typically showing higher expression.

• Recognizes formaldehyde-fixed epitope
• Reported application: Flow cytometric analysis
• Tested on stimulated normal human peripheral blood cells at 5 µL (0.06 µg)/test
• Light-sensitive: Protect vial and samples from light
• Fixation: Compatible with IC Fixation Buffer (Product # 00-8222) or 1-step Fix/Lyse Solution (Product # 00-5333)
• Excitation: 488–561 nm; Emission: 775 nm
• Laser Compatibility: Blue, Green, Yellow-Green
• Filtration: 0.2 µm post-manufacturing filtered

Target Information

LAG-3 is a 70-kDa surface glycoprotein of the Ig superfamily, homologous to CD4. It binds MHC class II with higher affinity than CD4 and negatively regulates T cell activation and proliferation. Expressed on activated T cells and NK cells; CD8+ T cells show higher expression. Coexpression with CD49b identifies Type 1 regulatory T cells (Tr1).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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