Thermo Fisher Scientific CDKN2A/p14ARF Polyclonal Antibody, FITC
CDKN2A/p14ARF 단백질을 인식하는 FITC 결합 토끼 다클론 항체. Western blot, IHC, ELISA, IP에 적합. 인간, 마우스, 설치류, 영장류 반응성. 형광 검출용으로 사용되며, 세포주기 및 종양 억제 연구에 활용 가능.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:1,000 |
| Immunohistochemistry (IHC) | 1:50–1:250 |
| ELISA | 1:10,000 |
| Immunoprecipitation (IP) | 1:50–1:250 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Non-human primate, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | N-epitope synthetic peptide corresponding to unique amino acid sequence on p14ARF protein |
| Conjugate | FITC |
| Excitation / Emission Max | 498 / 517 nm |
| Form | Liquid |
| Concentration | 0.5–1.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | Proprietary buffer, pH 7.4–7.8, with 0.5% BSA, 30% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C, store in dark |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
The INK4a locus on chromosome 9p21 is frequently affected in human tumors. It encodes two structurally distinct tumor suppressor proteins, p16INK4a and the alternative reading frame protein, ARF (p14ARF in human and P19ARF in mouse). These proteins interact with upstream tumor suppressors, retinoblastoma protein and p53, respectively. Both play roles in cellular senescence and tumor suppression through cell cycle regulation. p14ARF (or P19ARF in mouse) can induce G1 and G2 phase arrest depending on functional p53. Differences in ARF function between species may contribute to varying tumor susceptibility.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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