Thermo Fisher Scientific CDKN2A/p14ARF Polyclonal Antibody
CDKN2A/p14ARF 폴리클로날 항체로 Western blot, ELISA, IP 등에 사용 가능. 사람, 생쥐, 랫트 반응성. Rabbit IgG 기반, 액상 형태로 -20°C 보관. 세포주기 조절 및 종양 억제 연구에 적합.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:1,000 |
| ELISA | 1:10,000 |
| Immunoprecipitation (IP) | 1:50–1:250 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Mid-Region epitope synthetic peptide corresponding to unique amino acid sequence on p14ARF protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5–1.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | Proprietary buffer, pH 7.4–7.8, with 0.5% BSA, 30% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
The INK4a locus on chromosome 9p21 is frequently affected in human tumors. It encodes two structurally distinct tumor suppressor proteins, p16INK4a and the alternative reading frame protein, ARF (p14ARF in human and P19ARF in mouse).
These two proteins interact with upstream tumor suppressors, retinoblastoma protein and p53, respectively. Both play critical roles in cellular senescence, cell cycle regulation, and tumor suppression.
p14ARF (or P19ARF in mouse) can induce G1 and G2 phase arrest in a p53-dependent manner. The functional differences between mouse and human ARF proteins may contribute to species-specific tumor susceptibility.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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