Thermo Fisher Scientific CDKN2A/p14ARF Polyclonal Antibody, Biotin
CDKN2A/p14ARF 단백질을 인식하는 Thermo Fisher Scientific의 Biotin 결합 폴리클로날 항체. Western blot, ELISA, Immunoprecipitation에 적합하며, 사람·마우스·랫트 반응성. 종양 억제 및 세포주기 조절 연구용으로 사용.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:1,000 |
| ELISA | 1:10,000 |
| Immunoprecipitation (IP) | 1:50–1:250 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Mid-region synthetic peptide corresponding to unique amino acid sequence on p14ARF protein |
| Conjugate | Biotin |
| Form | Liquid |
| Concentration | 0.5–1.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | Proprietary buffer (pH 7.4–7.8) with 30% glycerol, 0.5% BSA |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
The INK4a locus on chromosome 9p21 is frequently affected in human tumors. It encodes two structurally distinct tumor suppressor proteins: p16INK4a and the alternative reading frame protein ARF (p14ARF in human and P19ARF in mouse).
These proteins interact with upstream regulators — retinoblastoma protein and p53 tumor suppressors — each playing a role in cellular senescence, cell cycle control, and tumor suppression.
p14ARF (or P19ARF in mouse) can induce both G1 and G2 phase arrest in a p53-dependent manner. The functional differences between mouse and human ARF proteins may contribute to species-specific tumor susceptibility.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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