Thermo Fisher Scientific Estrogen Receptor Alpha Polyclonal Antibody

Applications

Western Blot (WB)

• Tested Dilution: 1:2,000–1:10,000

Immunoprecipitation (IP)

• Tested Dilution: 2–10 µg/mg lysate

Product Specifications

Product Specific Information

The recommended shelf life for this product is 1 year from the date of receipt.

Target Information

Estrogen Receptors (ER) are members of the steroid/thyroid hormone receptor superfamily of nuclear receptors. The estrogen receptor is a ligand-activated transcription factor that, when bound to estrogen hormone, induces a conformational change allowing dimerization and binding to estrogen response elements (ERE) in DNA. When bound to EREs, ER can positively or negatively regulate gene transcription through the recruitment of coactivator or corepressor proteins.

There are two forms of the estrogen receptor, alpha and beta, encoded by separate genes (ESR1 and ESR2). Due to alternative RNA splicing, at least four estrogen receptor-alpha isoforms are known to exist:

• Isoform 1 (66 kDa)
• Isoform 2 (53 kDa)
• Isoform 3 (47 kDa)
• Isoform 4 (35 kDa)

Estrogen receptors are widely expressed in various tissues and are essential for sexual development and reproductive function. They also play roles in bone and other tissues. Estrogen receptors are involved in pathological processes including breast cancer, endometrial cancer, and osteoporosis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.