Thermo Fisher Scientific 4EBP1 Polyclonal Antibody
Rabbit polyclonal antibody targeting human 4EBP1 for WB and IHC applications. Recognizes residues 75–118 of 4EBP1. Suitable for mouse and rat cross-reactivity. Supplied as liquid, unconjugated form at 1 mg/mL, stored at 4 °C. Research use only.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000–1:6,000 |
| Immunohistochemistry (IHC) | 1:5,000–1:20,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:5,000–1:20,000 |
Product Specifications
| Property | Description |
|---|---|
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Region between residue 75 and the C-terminus (residue 118) of human eukaryotic translation initiation factor 4E binding protein 1 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Storage Conditions | 4 °C |
| Shipping Conditions | Wet ice |
Product Specific Information
- Recommended shelf life: 1 year from date of receipt
- For IHC, epitope retrieval with citrate buffer (pH 6.0) is recommended for FFPE tissue sections
- Based on 100% sequence identity, this antibody is predicted to react with Mouse and Rat
Target Information
This gene encodes a member of a family of translation repressor proteins. The protein interacts directly with eukaryotic translation initiation factor 4E (eIF4E), a limiting component of the multisubunit complex that recruits 40S ribosomal subunits to the 5′ end of mRNAs. Interaction with eIF4E inhibits complex assembly and represses translation. Phosphorylation of this protein in response to signals such as UV irradiation and insulin leads to its dissociation from eIF4E and activation of mRNA translation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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