Thermo Fisher Scientific LGR4 Monoclonal Antibody (6G8.B3.G5.C3)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:3,000

-

Immunohistochemistry (IHC)

5 µg/mL

-

ELISA (ELISA)

1:20,000-1:100,000

-

Immunoprecipitation (IP)

1:500-1:3,000

-

Product Specifications

Species Reactivity

Chimpanzee, Human, Rhesus monkey

Host/Isotype

Mouse / IgG2b, kappa

Class

Monoclonal

Type

Antibody

Clone

6G8.B3.G5.C3

Immunogen

This LGR4 antibody was produced by repeated immunizations with a synthetic peptide corresponding to an internal region of human LGR4 protein. The hybridoma was produced by fusing BALB/c mouse splenocytes and mouse myeloma SP2/O cells using conventional technology.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

0.02M potassium phosphate, pH 7.2, with 0.15M NaCl

Contains

0.01% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

Product Specific Information

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

This antibody is specific for human LGR4 protein. A BLAST analysis was used to suggest cross-reactivity with LGR4 from chimpanzee, orangutan and macaque based on 100% homology with the immunizing sequence. Cross-reactivity with LGR4 from other sources has not been determined.

Target Information

G protein-coupled receptors (GPCRs, or GPRs) contain 7 transmembrane domains and transduce extracellular signals through heterotrimeric G proteins. GPCR LGR4 is a member of this family (subfamily Orphan-A). Its expression has been documented in human brain, adrenal, heart, kidney, liver, skeletal muscle, ovary, pancreas, placenta, prostate, spleen, stomach, testis, thyroid, and spinal cord.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.