Thermo Fisher Scientific KGF Polyclonal Antibody, PeproTech
Recombinant Human KGF(FGF-7)에 특이적인 Rabbit Polyclonal Antibody로, Western Blot, ELISA, Neutralization, Immunostaining에 사용 가능. 항원 친화 크로마토그래피로 정제되었으며, Lyophilized 형태로 -20°C에서 보관. 연구용으로만 사용.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 0.1–0.2 µg/mL | - |
| ELISA | 0.5–2.0 µg/mL | 1 publication |
| Neutralization (Neu) | - | 1 publication |
| Immunostaining (IS) | - | 1 publication |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Published Species | Human, Rat |
| Host/Isotype | Rabbit |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | E.coli-derived Recombinant Human KGF (FGF-7) |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 0.1–1.0 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS |
| Contains | No preservative |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient |
| RRID | AB_2929747 |
Product Specific Information
Amino Acid Sequence of Recombinant Protein:
CNDMTPEQMA TNVNCSSPER HTRSYDYMEG GDIRVRRLFC RTQWYLRIDK RGKVKGTQEM KNNYNIMEIR TVAVGIVAIK GVESEFYLAM NKEGKLYAKK ECNEDCNFKE LILENHYNTY ASAKWTHNGG EMFVALNQKG IPVRGKKTKK EQKTAHFLPM AIT.
Preparation:
Produced from sera of rabbits immunized with highly pure Recombinant Human KGF (FGF-7). Anti-Human KGF (FGF-7)-specific antibody was purified by affinity chromatography employing an immobilized Human KGF (FGF-7) matrix.
Sandwich ELISA:
To detect Human KGF (FGF-7) by sandwich ELISA (using 100 µL/well antibody solution), a concentration of 0.5–2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Human KGF (FGF-7) (500-P19Bt) as a detection antibody, allows the detection of at least 0.2–0.4 ng/well of Recombinant Human KGF (FGF-7).
Western Blot:
To detect Human KGF (FGF-7) by Western Blot analysis, this antibody can be used at a concentration of 0.1–0.2 µg/mL. Used in conjunction with compatible secondary reagents, the detection limit for Recombinant Human KGF (FGF-7) is 1.5–3.0 ng/lane, under either reducing or non-reducing conditions.
Target Information
KGF(FGF-7)는 배아 발달, 세포 증식 및 분화 조절에 중요한 역할을 하며, 정상적인 분지형태형성(branching morphogenesis)에 필수적입니다. 각질형성세포(keratinocyte)에 작용하는 성장인자로, 정상 상피세포 증식의 주요 파라크린 조절 인자일 가능성이 있습니다.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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