Thermo Fisher Scientific KGF Polyclonal Antibody, PeproTech

Applications

Tested Dilution

Publications

Western Blot (WB)

0.1-0.2 µg/mL

-

ELISA (ELISA)

0.5-2.0 µg/mL

View 1 publication 1 publication

Neutralization (Neu)

-

View 1 publication 1 publication

Immunostaining (IS)

-

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human

Published species

Human, Rat

Host/Isotype

Rabbit

Class

Polyclonal

Type

Antibody

Immunogen

E.coli-derived Recombinant Human KGF (FGF-7) if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:KGF,uniProtId:P21781-1,ncbiNodeId:9606,antigenRange:32-194,antigenLength:194,antigenImageFileName:500-P19-100UG_KGF_P21781-1_Rabbit.svg,antigenImageFileNamePDP:500-P19-100UG_KGF_P21781-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

0.1-1.0 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS

Contains

no preservative

Storage conditions

-20°C

Shipping conditions

Ambient

RRID

AB_2929747

Product Specific Information

AA Sequence of recombinant protein: CNDMTPEQMA TNVNCSSPER HTRSYDYMEG GDIRVRRLFC RTQWYLRIDK RGKVKGTQEM KNNYNIMEIR TVAVGIVAIK GVESEFYLAM NKEGKLYAKK ECNEDCNFKE LILENHYNTY ASAKWTHNGG EMFVALNQKG IPVRGKKTKK EQKTAHFLPM AIT.

Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human KGF (FGF-7). Anti-Human KGF (FGF-7)-specific antibody was purified by affinity chromatography employing an immobilized Human KGF (FGF-7) matrix.

Sandwich ELISA: To detect Human KGF (FGF-7) by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.5-2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Human KGF (FGF-7) (500-P19Bt) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human KGF (FGF-7).

Western Blot: To detect Human KGF (FGF-7) by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Human KGF (FGF-7) is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

Target Information

Plays an important role in the regulation of embryonic development, cell proliferation and cell differentiation. Required for normal branching morphogenesis. Growth factor active on keratinocytes. Possible major paracrine effector of normal epithelial cell proliferation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.