Thermo Fisher Scientific IL-20 Polyclonal Antibody, PeproTech

Applications

Product Specifications

Product Specific Information

Amino Acid Sequence of Recombinant Protein:
MLKTLNLGSC VIATNLQEIR NGFSEIRGSV QAKDGNIDIR ILRRTESLQD TKPANRCCLL RHLLRLYLDR VFKNYQTPDH YTLRKISSLA NSFLTIKKDL RLCHAHMTCH CGEEAMKKYS QILSHFEKLE PQAAVVKALG ELDILLQWME ETE.

Preparation:
Produced from sera of goats immunized with highly pure Recombinant Human IL-20. Anti-Human IL-20-specific antibody was purified by affinity chromatography employing an immobilized Human IL-20 matrix.

Sandwich ELISA:
To detect Human IL-20 by sandwich ELISA (using 100 µL/well antibody solution), a concentration of 0.5–2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Human IL-20 (500-P190GBt) as a detection antibody, allows the detection of at least 0.2–0.4 ng/well of Recombinant Human IL-20.

Western Blot:
To detect Human IL-20 by Western Blot analysis, this antibody can be used at a concentration of 0.1–0.2 µg/mL. When used with compatible secondary reagents, the detection limit for Recombinant Human IL-20 is 1.5–3.0 ng/lane, under either reducing or non-reducing conditions.

Target Information

Cytokines that signal through Class II receptors form a distinct family that includes the interferons and interleukin 10 (IL-10). Several IL-10 homologs, including AK155, IL-19, IL-20, IL-22, and IL-24, define a cytokine subfamily. IL-20 belongs to the IL-10 family and plays a role in skin inflammation and the development of hematopoietic cells. These cytokines display pro-inflammatory activities in different tissues, including skin.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.