Thermo Fisher Scientific IL-21 Polyclonal Antibody, PeproTech
Rabbit에서 생산된 Human IL-21 특이적 폴리클로날 항체로, Western blot, ELISA 등 다양한 응용에 사용 가능. 항원 친화 크로마토그래피로 정제되었으며, IL-21 관련 면역학 연구에 적합. 동결건조 형태로 -20°C에서 보관.
- 카탈로그번호
- 500-P191-xxx (3개 옵션)
- 판매단위
- pk
카탈로그
3개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific IL-21 Polyclonal Antibody, PeproTech
Thermo Fisher Scientific IL-21 Polyclonal Antibody, PeproTech
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 0.1–0.2 µg/mL | View 1 publication |
| ELISA | 0.5–2.0 µg/mL | - |
| Neutralization (Neu) | - | View 1 publication |
| In vitro Assay (IV) | - | View 2 publications |
| Immunostaining (IS) | - | View 1 publication |
Product Specifications
| Property | Description |
|---|---|
| Species Reactivity | Human |
| Published Species | Human |
| Host/Isotype | Rabbit |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | E.coli-derived Recombinant Human IL-21 |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 0.1–1.0 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS |
| Contains | No preservative |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient |
| RRID | AB_2929748 |
Product Specific Information
AA Sequence of recombinant protein:
MQDRHMIRMR QLIDIVDQLK NYVNDLVPEF LPAPEDVETN CEWSAFSCFQ KAQLKSANTG NNERIINVSI KKLKRKPPST NAGRRQKHRL TCPSCDSYEK KPPKEFLERF KSLLQKMIHQ HLSSRTHGSE DS
Preparation:
Produced from sera of rabbits immunized with highly pure Recombinant Human IL-21. Anti-Human IL-21-specific antibody was purified by affinity chromatography employing an immobilized Human IL-21 matrix.
Sandwich ELISA:
For detection of Human IL-21 by sandwich ELISA (using 100 µL/well antibody solution), a concentration of 0.5–2.0 µg/mL is required. In conjunction with PeproTech Biotinylated Anti-Human IL-21 (500-P191Bt) as a detection antibody, it enables detection of at least 0.2–0.4 ng/well of Recombinant Human IL-21.
Western Blot:
For detection of Human IL-21 by Western blot analysis, use at 0.1–0.2 µg/mL. With compatible secondary reagents, the detection limit for Recombinant Human IL-21 is 1.5–3.0 ng/lane under reducing or non-reducing conditions.
Target Information
IL-21 is a 17 kDa immunomodulatory cytokine produced mainly by Natural Killer (NKT), T helper (Th17), and T follicular helper (TFH) cells.
In TFH cells, IL-21 induces autocrine signaling through IL-21R and STAT3, leading to transcriptional activation by Bcl6. Similar to IFN-γ for Th1 and IL-4 for Th2, IL-21 is essential for TFH cell development and function.
IL-21 promotes T cell-dependent B cell differentiation into plasma and memory cells, stimulates IgG production, and induces apoptosis in naive B cells.
In Th17 cells, IL-21 enhances IL-23R expression via STAT3, IRF4, and RORγt, supporting Th17 maturation and maintenance. It downregulates Foxp3 expression and is linked to colitis models, where IL-21 deficiency confers protection.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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