Thermo Fisher Scientific RAP1GAP Polyclonal Antibody

Applications

Immunohistochemistry (Paraffin) (IHC (P))

• Tested Dilution: 1:200–1:500

Product Specifications

Product Specific Information

• Immunogen sequence:
  IGIENIQEVQ EKRESPPAGQ KTPDSGHVSQ EPKSENSSTQ SSPEMPTTKN RAETAAQRAE ALKDFSRSSS SASSFASVVE ETEGVDGEDT GLESVSSSGT P
• Highest antigen sequence identity to orthologs:
  Mouse - 92%, Rat - 91%

Target Information

Rap1GAP specifically stimulates GTP hydrolytic activity of the monomeric G protein Rap1. Physical interaction between G alpha z (a member of the Gi family of trimeric G proteins) and Rap1GAP blocks the ability of regulators of G protein signaling to stimulate GTP hydrolysis of the alpha subunit, and attenuates the ability of activated G alpha z to inhibit adenylyl cyclase.
Rap1GAP is expressed in the brain, kidney, and pancreas and may act as a signal integrator to coordinate Gz signaling and Rap1 signaling in cells.
A novel isoform, Rap1GAPII, binds specifically to G alpha z. Stimulation of the Gi-coupled M2 Muscarinic receptor translocates Rap1GAPII from the cytosol to the membrane and decreases GTP-bound Rap1, resulting in ERK/MAPK activation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.