Thermo Fisher Scientific Involucrin Recombinant Rabbit Monoclonal Antibody (IVRN, 2113R)
Involucrin 단백질을 인식하는 토끼 단클론 항체로, IHC(P) 검증 완료. 사람, 개, 비인간 영장류, 돼지 반응성. HEK293 세포 발현, Protein A/G 정제. 비결합형 액상 포맷, 4°C 보관. 연구용 전용.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Immunohistochemistry (Paraffin) (IHC (P)) | 1–2 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Dog, Human, Non-human primate, Pig |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | IVRN, 2113R |
| Immunogen | Recombinant human full-length Involucrin protein (IVRN) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 200 µg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4, with 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- This antibody does not react with Mouse.
- For immunohistochemistry (PFA fixed): incubate antibody for 30 minutes at room temperature.
- Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 minutes at 95°C, followed by cooling at room temperature for 20 minutes.
Target Information
Involucrin is a component of the keratinocyte crosslinked envelope, localized in the cytoplasm and crosslinked to membrane proteins by transglutaminase. It is expressed in stratified squamous epithelia, including the cornea. In normal epidermis, it appears in the upper spinous layers, and in keratinocyte cultures, it is expressed by cells that have left the basal layer. Involucrin expression is abnormal in squamous cell carcinomas and premalignant lesions, and reduced in severe dysplasias of the larynx and cervix. The gene is mapped to chromosome 1q21.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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