Thermo Fisher Scientific Involucrin Recombinant Rabbit Monoclonal Antibody (IVRN, 2113R)

Applications

• Immunohistochemistry (Paraffin) (IHC (P)): 1–2 µg/mL

Product Specifications

Product Specific Information

• This antibody does not react with Mouse.
• For Immunohistochemistry (PFA fixed): incubate antibody for 30 min at room temperature.
• Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA (pH 9.0) for 45 min at 95°C, followed by cooling at room temperature for 20 minutes.

Target Information

Involucrin, a component of the keratinocyte crosslinked envelope, is found in the cytoplasm and crosslinked to membrane proteins by transglutaminase. It is expressed in a range of stratified squamous epithelia, including the cornea. In normal epidermis, involucrin is first expressed in the upper spinous layers, and in keratinocyte cultures, it is expressed by all cells that have left the basal layer.
Involucrin expression is abnormal in squamous cell carcinomas and premalignant lesions and is reduced in severe dysplasias of the larynx and cervix. Its gene is mapped to chromosome 1q21, among calpactin I light chain, trichohyalin, profillaggrin, loricrin, and calcyclin.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.