Thermo Fisher Scientific Phospho C-Jun (Thr91, Thr93) Monoclonal Antibody (C-J 4C4/1)
인산화된 c-Jun (Thr91, Thr93)을 검출하는 고특이성 단클론 항체. Western blot, IHC, ICC, Flow cytometry 등 다양한 응용 가능. Human 및 Rat 반응성. Protein A 정제, 보존제 없음, 연구용 전용.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1–2 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | Assay-dependent |
| Immunohistochemistry (PFA fixed) (IHC (PFA)) | 1–2 µg/mL |
| Immunocytochemistry (ICC/IF) | 1–2 µg/mL |
| Flow Cytometry (Flow) | 1–2 µg/10⁶ cells |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Rat |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | C-J 4C4/1 |
| Immunogen | c-Jun protein phosphorylated at T91 and T93 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS, pH 7.4 |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
c-Jun is a transcription factor that recognizes and binds to the enhancer heptamer motif 5’-TGA[CG]TCA-3’. It promotes activity of NR5A1 when phosphorylated by HIPK3, leading to increased steroidogenic gene expression upon cAMP signaling pathway stimulation. It is involved in activated KRAS-mediated transcriptional activation of USP28 in colorectal cancer (CRC) cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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