Thermo Fisher Scientific MCL1 Monoclonal Antibody (OTI2E11), TrueMAB
MCL1 단백질을 특이적으로 인식하는 Mouse monoclonal antibody로, WB, ICC/IF, Flow Cytometry에 적합합니다. Lyophilized 형태로 제공되며, 재구성 후 약 1 mg/mL 농도로 사용 가능합니다. 인간 MCL1 발현 연구 및 세포사멸 관련 연구에 유용합니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000 |
| Immunocytochemistry (ICC/IF) | 1:100 |
| Flow Cytometry (Flow) | 1:100 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI2E11 |
| Immunogen | Full length human recombinant protein of human MCL1 produced in HEK293T cell |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL of distilled water to obtain a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, it is recommended to perform an additional desalting step using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).
Target Information
MCL1 (Myeloid cell leukemia-1) is a member of the Bcl-2 family involved in differentiation and maintenance of cell viability. Isoform 1 inhibits apoptosis, while isoform 2 promotes it. MCL1 expression increases upon exposure to DNA-damaging agents, correlating with upregulation of GADD45 and Bax and downregulation of bcl-2.
MCL1 is associated with mitochondria and helps maintain cell viability under apoptotic conditions. It is essential for embryogenesis and the development of B and T lymphocytes. MCL1 exists as two isoforms, MCL1L and MCL1S; the latter promotes cell death when overexpressed.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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