Thermo Fisher Scientific MCL1 Monoclonal Antibody (OTI4B7), TrueMAB
인간 MCL1 단백질을 인식하는 Mouse IgG1 단클론 항체로 Western blot, IHC, ICC/IF에 사용 가능. 동결건조 형태로 제공되며 PBS buffer에 trehalose 포함. 세포 생존 및 apoptosis 연구용으로 적합.
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Thermo Fisher Scientific MCL1 Monoclonal Antibody (OTI4B7), TrueMAB
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50 |
| Immunocytochemistry (ICC/IF) | 1:100 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI4B7 |
| Immunogen | Full length human recombinant protein of human MCL1 produced in HEK293T cells |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).
Target Information
MCL1 (Myeloid cell leukemia-1) is a member of the Bcl-2 family involved in cell viability and apoptosis regulation.
Isoform 1 inhibits apoptosis, while isoform 2 promotes it. MCL1 expression increases in response to DNA damage and is associated with mitochondrial function.
It plays a critical role in embryogenesis and lymphocyte development. Overexpression of the shorter isoform (MCL1S) promotes cell death.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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