Thermo Fisher Scientific MCL1 Monoclonal Antibody (OTI9C9), TrueMAB
MCL1 단백질을 표적하는 Mouse monoclonal antibody로, WB, IHC, ICC/IF에 사용 가능. 고순도 Affinity chromatography 정제, Lyophilized 형태로 제공. 인간 MCL1 전체 길이 재조합 단백질을 면역원으로 사용하며, 연구용으로 적합.
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Thermo Fisher Scientific MCL1 Monoclonal Antibody (OTI9C9), TrueMAB
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50 |
| Immunocytochemistry (ICC/IF) | 1:100 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host/Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI9C9 |
| Immunogen | Full length human recombinant protein of human MCL1 produced in HEK293T cell |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments using this carrier-free antibody, perform an additional desalting step using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).
Target Information
MCL1 (Myeloid cell leukemia-1) is a member of the Bcl-2 family involved in cell viability maintenance, differentiation, and apoptosis regulation. Isoform 1 inhibits apoptosis, while isoform 2 promotes it. MCL1 expression increases in response to DNA-damaging agents and is associated with mitochondrial localization. It plays a crucial role in embryogenesis and lymphocyte development. Overexpression of the smaller isoform (MCL1S) promotes cell death, contrasting with the protective role of MCL1L.
For Research Use Only. Not for use in diagnostic procedures or resale without express authorization.
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