Thermo Fisher Scientific PHD1 Polyclonal Antibody
PHD1 단백질을 인식하는 Thermo Fisher Scientific의 폴리클로날 항체로, Western blot, ICC/IF, IP, ChIP 등 다양한 응용에 적합. 높은 순도(>95%)와 안정적인 로트 간 일관성을 제공하며, 인간, 생쥐, 랫트 시료에 반응. 연구용으로만 사용 가능.
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Applications
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:1,000–1:5,000 | - |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 | - |
| Immunoprecipitation (IP) | 1:20–1:50 | - |
| ChIP assay (ChIP) | 1:20–1:50 | - |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide of human hydroxylase PHD1 (EGLN2) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2717291 |
Product Specific Information
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. These systems are developed by cloning specific antibody DNA sequences from immunoreactive rabbits, followed by screening individual clones to select optimal candidates for production.
Advantages include:
- Better specificity and sensitivity
- Lot-to-lot consistency
- Animal origin-free formulations
- Broader immunoreactivity due to larger rabbit immune repertoire
Purity is >95% by SDS-PAGE.
Target Information
Prolyl hydroxylase domain proteins HIF PHD1, HIF PHD2, and HIF PHD3 hydroxylate HIF-alpha subunits, which are key regulators of oxygen homeostasis.
HIF PHD1 is widely expressed, with highest levels in testis, and acts as a cellular oxygen sensor important in cell growth regulation. It can localize to both nucleus and cytoplasm and is found in hormone-responsive tissues such as mammary, ovarian, and prostate epithelium (normal and cancerous).
HIF PHD1 is encoded by EGLN2, located on chromosome 19q13.3.
For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.
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