Thermo Fisher Scientific ASH2L Recombinant Rabbit Monoclonal Antibody (2D9)
Thermo Fisher의 ASH2L Recombinant Rabbit Monoclonal Antibody (2D9)는 인간, 마우스, 랫트 시료에 반응하며 높은 특이성과 일관성을 제공합니다. Western blot, IHC, ICC 등 다양한 응용에 적합하며, 동물 유래 성분이 없는 포뮬레이션으로 재현성이 우수합니다. 연구용으로만 사용 가능합니다.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000–1:5,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:200–1:500 |
| Immunocytochemistry (ICC/IF) | 1:200–1:500 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | 2D9 |
| Immunogen | Recombinant ASH2L |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS, pH 7.2, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2717296 |
Product Specific Information
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The antibody DNA sequences from immunoreactive rabbits are cloned, and individual clones are screened to select optimal candidates for production.
Advantages include:
- Superior specificity and sensitivity
- Lot-to-lot consistency
- Animal origin-free formulations
- Broader immunoreactivity due to the larger rabbit immune repertoire
Target Information
The human ASH2L gene encodes a 628 amino acid protein (isoform 1, ASH2L1) with a nuclear localization signal and PHD finger motif, suggesting a role as a transcription regulator.
- Predicted molecular weight: 68.7 kDa
- Alternative isoforms: 60 kDa and 56.5 kDa
- Homology: 60% to Drosophila ash2, 90% to mouse ASH2L
- Expression: Predominantly in adult heart and testis, and fetal lung and liver
- Low expression in adult lung, liver, kidney, prostate, and peripheral leukocytes
- In leukemia cell lines (e.g., K562, Hel, Dami), ASH2L expression varies with differentiation inducers such as phorbol ester and hemin, indicating a role in hematopoiesis and leukemia.
- Suggested interactions with beta-tubulin and beta-catenin.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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