Histidine
Details
Histidine discs are used for amino acid decarboxylation test. To determine amino acid decarboxylation, the Serine disc (DD054) is added in the Decarboxyalse Broth Base, Moeller (M393)which is used as a negative control for studying decarboxylation or as a base for the addition of amino acids. The test organismisinoculated into the broth containing the Serine disc (DD054). The inoculated tubes are overlaid with sterile mineral oil andincubated at 35-37°C for up to 4 days. Positive Test: Colour of the medium changes from yellow to purple. Negative Test: Colour of the medium changes to yellow or there is no change
