Thermo Fisher Scientific DYKDDDDK-tag Polyclonal Antibody
Rabbit polyclonal antibody recognizing DYKDDDDK (FLAG) tag for detection of tagged recombinant proteins. Suitable for WB, ICC/IF, Flow Cytometry, and ELISA. Lyophilized form, reconstitutable to 0.5 mg/mL. For research use only.
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Applications
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 0.1–1.0 µg/mL | - |
| Immunocytochemistry (ICC/IF) | 5–20 µg/mL | - |
| Flow Cytometry (Flow) | 1–3 µg / 1 × 10⁶ cells | - |
| ELISA | 0.01–0.05 µg/mL | - |
| Miscellaneous PubMed (Misc) | – | View 16 publications |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Tag |
| Published Species | Not Applicable |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide DYKDDDDK (Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys) conjugated to KLH |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Purification | Affinity chromatography |
| Storage Buffer | PBS |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C or -80°C if preferred |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
Reconstitute the lyophilized antibody with deionized water (or equivalent) to a final concentration of 0.5 mg/mL.
The DYKDDDDK peptide is a small epitope that does not interfere with bioactivity or biodistribution of recombinant proteins. It is widely used as a general epitope tag in expression vectors.
DYKDDDDK-tag Antibody, pAb, Rabbit can be used to detect DYKDDDDK-tagged proteins.
Target Information
The DYKDDDDK tag, also known as FLAG tag, is a short peptide sequence used for protein purification, detection, and localization.
It consists of eight amino acids: Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys, and is typically fused to the N- or C-terminus of a protein of interest.
This tag allows for easy identification and isolation using anti-FLAG antibodies.
The FLAG tag is widely utilized in molecular biology and biochemistry for:
- Protein-protein interaction studies
- Protein expression analysis
- Protein localization studies
The FLAG tag is more hydrophilic than other common epitope tags, reducing the risk of denaturation or inactivation of fused proteins.
FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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