Thermo Fisher Scientific TdT/DNA Nucleotidylexotransferase Recombinant Rabbit Monoclonal Antibody (DNTT, 4506R)
Recombinant rabbit monoclonal antibody recognizing TdT (DNTT, 4506R). Validated for IHC-P applications. High specificity for human TdT with protein A/G purification. Supplied as liquid form, 200 µg/mL, stored at 4°C.
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Applications
Tested Application
- Immunohistochemistry (Paraffin) (IHC-P)
Recommended Dilution
- 1–2 µg/mL
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | DNTT, 4506R |
| Immunogen | Synthetic peptide corresponding to residues within aa1–100 of TdT |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 200 µg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4, with 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- For IHC (PFA-fixed): Incubate antibody for 30 minutes at room temperature.
- Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 minutes at 95°C, followed by cooling at room temperature for 20 minutes.
Target Information
Terminal Deoxynucleotidyl Transferase (TdT) is a DNA polymerase located in the cell nucleus that catalyzes the polymerization of deoxynucleotides at the 3′ hydroxyl ends of oligo- or polydeoxynucleotide initiators and functions without a template.
TdT is considered a highly specific marker for the diagnosis and classification of acute lymphoblastic lymphoma/leukemias. Determination of TdT expression is particularly useful for differentiating histologically between lymphoblastic lymphoma and Burkitt’s lymphoma.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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