Thermo Fisher Scientific Rat anti-Mouse IgG1 Secondary Antibody, Super Bright 436, eBioscience

Applications and Tested Dilution

• Application: Flow Cytometry (Flow)
• Tested Dilution: 1.0 µg/test

Product Specifications

Additional Available Formats

• APC
• Biotin
• eFluor 450
• eFluor 570
• eFluor 615
• eFluor 660
• FITC
• HRP
• PE
• PE-Cyanine7
• PE-eFluor 610
• PerCP-eFluor 710
• Super Bright 600
• Super Bright 780
• Custom conjugation available on request

Product Specific Information

The monoclonal antibody M1-14D12 recognizes mouse IgG1 antibodies and is suitable as a secondary reagent in flow cytometry and microscopy.
It does not cross-react with other mouse isotypes or rat immunoglobulins.

• Applications Reported: Flow cytometric analysis
• Applications Tested: Tested on normal human peripheral blood cells at ≤1.0 µg/test
  • A test is defined as the amount of antibody required to stain a 100 µL cell sample
  • Cell number can range from 10⁵ to 10⁸ cells/test
  • Optimal concentration should be determined empirically

Fluorochrome Information:
Super Bright 436 is excited by the violet laser (405 nm) and emits at 436 nm.
Recommended filter: 450/50 bandpass or equivalent. Ensure instrument compatibility.

When using multiple Super Bright dye-conjugated antibodies, use Super Bright Complete Staining Buffer (Product # SB-4401) to minimize non-specific polymer interactions.

• Excitation: 405 nm
• Emission: 436 nm
• Laser: Violet

Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.

Target Information

Anti-Mouse secondary antibodies are affinity-purified and specifically recognize mouse immunoglobulins.
They are widely used for detection, sorting, and purification of mouse antibodies.
Secondary antibodies enhance assay versatility and sensitivity by enabling multiple detection systems (HRP, AP, fluorescence) and signal amplification.
Typically, they are produced by immunizing host animals with pooled immunoglobulins from the target species and further purified or modified for specificity.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

제품 이미지