Invitrogen™

Anza™ 36 Eco31I

5G G T C T C N1 ↓ 33C C A G A G N5 ↑ 5Invitrogen™ Anza™자세히 알아보기

5G G T C T C N1 ↓ 3
3C C A G A G N5 ↑ 5

Invitrogen™ Anza™ 36 Eco31I is a restriction enzyme that cuts DNA at this recognition site: GGTCTC(1/5), completely digesting the DNA in 15 minutes at 37°C. Isoschizomers include: BsaI, Bso31I, BspTNI.

For superior convenience, a single buffer and protocol are used with all Anza™ restriction enzymes. The single Anza buffer allows multiple restriction enzymes to be used simultaneously in one reaction. This helps eliminate the need for sequential digests and the DNA clean-up necessary when changing buffers. Additionally, the Anza buffer and restriction enzymes have been designed for flexibility in digestion times. Digestions of up to 16 hours can be carried out without star activity.

• Convenient—all Anza enzymes are 100% functional in the single Anza buffer
• Fast—complete digestion in 15 minutes
• Flexible—overnight digestion without star activity
• Simple—a single digestion protocol for all DNA types
• Complete system—incorporates Anza DNA Modifying Enzymes for a complete cloning experience

Anza DNA Modifying Enzymes include the Anza T4 DNA Ligase Master Mix, Anza Alkaline Phosphatase Kit, Anza T4 PNK Kit, Anza DNA Blunt End Kit, and Anza DNA End Repair Kit. Use these enzymes for a complete cloning workflow.

Anza Red Buffer, which contains a density gradient and tracking dyes, is supplied for added convenience. Digestion reactions are as efficient in the Anza Red Buffer as in the Anza Buffer. Use of the Anza Red Buffer helps reduce pipetting steps when preparing multiple digestion reactions and a subsequent step includes agarose gel electrophoresis. For applications that require product analysis by fluorescence excitation, the colorless Anza Buffer without the tracking dyes is recommended.

사양

배송 조건Approved for shipment on Wet or Dry Ice

메틸화 민감도CpG Methylation-Sensitive, Not CpG Methylation-Sensitive, Not Dam Methylation-Sensitive

효소Eco31I

함께 사용가능한 버퍼Anza™ 10X Red Buffer, Anza™ 10X Buffer

열 불활성화에 대한 민감성Yes

최적 반응 온도37°C

Type IIS REYes

수량800 units

제품 유형Restriction Enzyme Cloning System

연구 카테고리Traditional Cloning

제품라인Anza™

Unit SizeEach