Thermo Fisher Scientific HGF Polyclonal Antibody
Thermo Fisher Scientific의 HGF Polyclonal Antibody는 마우스 HGF에 반응하는 Rabbit IgG 항체로, WB에 적합합니다. 합성 펩타이드 면역원으로 제작되었으며, 항원 친화 크로마토그래피로 정제되었습니다. -20°C에서 보관하며 연구용으로 사용됩니다.
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Applications
Western Blot (WB)
- Tested Dilution: 0.1–0.5 µg/mL
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthetic peptide corresponding to a sequence at the N-terminus of mouse HGF (33–64aa QKKRRNTLHEFKKSAKTTLTKEDPLLKIKTKK) |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 500 µg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS with 4 mg trehalose |
| Contains | 0.05 mg sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Wet ice |
| RRID | AB_2746477 |
Product Specific Information
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Target Information
Hepatocyte growth factor (HGF) regulates cell growth, motility, and morphogenesis through activation of a tyrosine kinase signaling cascade after binding to the c-Met receptor. Secreted by mesenchymal cells, HGF acts as a multifunctional cytokine mainly on epithelial cells. It stimulates mitogenesis, cell motility, and matrix invasion, playing a key role in angiogenesis, tumorigenesis, and tissue regeneration.
HGF is secreted as an inactive single polypeptide and cleaved by serine proteases into a 69-kDa alpha-chain and a 34-kDa beta-chain, forming an active heterodimer via a disulfide bond. It belongs to the plasminogen subfamily of S1 peptidases but lacks detectable protease activity. Alternative splicing generates multiple transcript variants encoding different isoforms.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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