Thermo Fisher Scientific TNF alpha Monoclonal Antibody (MP6-XT22), Brilliant Violet 421, eBioscience

Thermo Fisher Scientific TNF alpha Monoclonal Antibody (MP6-XT22), Brilliant Violet 421, eBioscience

Applications

• Flow Cytometry (Intracellular staining)

Tested Dilution: 1.0 µg/test

Product Specifications

Available Formats

• Unconjugated
• Alexa Fluor 488
• Alexa Fluor 561
• Alexa Fluor 660
• APC
• Brilliant Violet 480
• Brilliant Violet 605
• Brilliant Violet 650
• Brilliant Violet 786
• eFluor 450
• FITC
• Functional Grade
• PE
• PE-Cyanine7
• PE-eFluor 610
• PerCP-eFluor 710
• Request custom conjugation available

Product Specific Information

The MP6-XT22 antibody reacts with mouse tumor necrosis factor-alpha (TNF alpha), a 17 kDa cytokine produced by monocytes, macrophages, neutrophils, NK cells, and CD4(+) T cells. TNF alpha exhibits cytolytic activity against various tumor cells and plays a key role in immune regulation. It forms dimers and trimers and also exists in a 26 kDa membrane-bound form.

Applications Reported: Intracellular staining followed by flow cytometric analysis.
Applications Tested: Tested using mouse splenocytes and the Intracellular Fixation & Permeabilization Buffer Set (Product # 88-8824-00). Recommended ≤ 1.0 µg per test (100 µL final volume). Cell number may range from 10⁵ to 10⁸ cells/test. Antibody should be titrated for optimal performance.

Brilliant Violet™ 421 (BV421) emits at 423 nm and is compatible with cytometers equipped with a violet (405 nm) laser. Ensure instrument capability for BV421 detection.

When using multiple polymer dye-conjugated antibodies, use Super Bright Complete Staining Buffer (Product # SB-4401-42) or Brilliant Stain Buffer™ (Product # 00-4409-75) to minimize non-specific interactions.

Excitation: 407 nm
Emission: 423 nm
Laser: Violet Laser

BRILLIANT VIOLET™ is a trademark or registered trademark of Becton, Dickinson and Company or its affiliates and is used under license. Powered by Sirigen™.

Target Information

TNF alpha is a multifunctional proinflammatory cytokine belonging to the TNF superfamily. Secreted primarily by macrophages, it binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR receptors. TNF alpha regulates immune cells, proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. It forms multimers but shows a single 17 kDa band under non-reducing SDS-PAGE conditions. Mouse knockout studies suggest its neuroprotective role, and it induces tumor necrosis when injected into tumor-bearing mice. TNF alpha also mediates immune responses to bacterial, viral, parasitic, and fungal infections.

For Research Use Only. Not for use in diagnostic procedures or resale without authorization.

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