Thermo Fisher Scientific IL-12/IL-23 p40 Monoclonal Antibody (C8.6), Brilliant Violet 421, eBioscience

Thermo Fisher Scientific IL-12/IL-23 p40 Monoclonal Antibody (C8.6), Brilliant Violet 421, eBioscience

Applications

• Flow Cytometry (intracellular staining)

Tested Dilution

• 5 µL (0.25 µg)/test

Product Specifications

Additional Formats

• Unconjugated
• Alexa Fluor 488
• Biotin
• Brilliant UV 395
• eFluor 450
• eFluor 660
• Functional Grade
• PE
• Request custom conjugation

Product Specific Information

The C8.6 antibody reacts with the p40 subunit of human IL-12 p70 and human IL-23.
IL-12 is a 70 kDa heterodimeric cytokine composed of p40 and p35 subunits, secreted by activated monocytes, macrophages, and dendritic cells.
IL-23 is a related heterodimer composed of disulfide-linked p40 and p19 subunits.

Applications Reported:
Used for intracellular staining followed by flow cytometric analysis.

Applications Tested:
Pre-diluted and tested by intracellular staining and flow cytometric analysis of normal human peripheral blood cells using the Intracellular Fixation & Permeabilization Buffer Set (Product #88-8824-00).
Recommended usage: 5 µL (0.25 µg) per test (100 µL final volume, 10⁵–10⁸ cells/test).

Fluorochrome Information:
Brilliant Violet™ 421 (BV421) emits at 423 nm and is intended for use with a violet (405 nm) laser.
Ensure cytometer compatibility with this fluorochrome.

Staining Recommendation:
When using multiple polymer dye-conjugated antibodies, use Super Bright Complete Staining Buffer (Product #SB-4401-42) or Brilliant Stain Buffer™ (Product #00-4409-75) to minimize non-specific polymer interactions.

Excitation/Emission/Laser:
407 nm / 423 nm / Violet Laser

Trademark Notice:
BRILLIANT VIOLET™ is a trademark or registered trademark of Becton, Dickinson and Company or its affiliates, and is used under license. Powered by Sirigen.™

Target Information

IL-12 is produced by dendritic cells, neutrophils, macrophages, and lymphoblastoid cells upon antigenic stimulation.
IL-12 is composed of IL-12A (p35) and IL-12B (p40) subunits.
IL-23 shares IL-12B with IL-12 and includes IL-23A (p19).
IL-12 enhances endothelial adhesion molecule-1 expression and directs leukocytes to tumor sites, promoting ischemic-necrotic tumor responses.
IL-23 stimulates memory T-cells and is essential for Th17 cell function, neutrophil chemokine induction, and pro-inflammatory cytokine regulation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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