Thermo Fisher Scientific IL-2 Monoclonal Antibody (MQ1-17H12), Brilliant Violet 421, eBioscience

Thermo Fisher Scientific IL-2 Monoclonal Antibody (MQ1-17H12), Brilliant Violet 421, eBioscience

Applications and Tested Dilution

• Flow Cytometry (Intracellular staining)
  Recommended use: 5 µL (0.5 µg)/test

Product Specifications

Available Formats

• Unconjugated
• Alexa Fluor 532
• APC
• Brilliant UV 395 / 615 / 737
• Brilliant Violet 480
• eFluor 450
• FITC
• PE / PE-Cyanine7 / PE-eFluor 610
• PerCP-eFluor 710
• Custom conjugation available

Product Specific Information

The MQ1-17H12 antibody reacts with human interleukin-2 (IL-2), a 17 kDa T cell growth factor and major immunoregulatory cytokine. It is a non-neutralizing antibody suitable for intracellular staining followed by flow cytometric analysis.
This antibody has been tested using normal human peripheral blood cells with the Intracellular Fixation & Permeabilization Buffer Set (Product # 88-8824-00) according to the two-step protocol for intracellular (cytoplasmic) proteins.

• Recommended use: 5 µL (0.5 µg) per test (100 µL final volume)
• Suitable for 10⁵–10⁸ cells/test
• Excitation: 407 nm; Emission: 423 nm; Laser: Violet Laser

For optimal results when using multiple polymer dye-conjugated antibodies (e.g., Brilliant Violet™, Brilliant Ultra Violet™), use Super Bright Complete Staining Buffer (Product # SB-4401-42) or Brilliant Stain Buffer™ (Product # 00-4409-75) to minimize non-specific polymer interactions.

BRILLIANT VIOLET™ is a trademark of Becton, Dickinson and Company or its affiliates, used under license. Powered by Sirigen™.

Target Information

Interleukin 2 (IL-2) is a key immunomodulatory cytokine involved in:

• Proliferation of activated T cells
• Differentiation of B cells, NK cells, monocytes, and macrophages
• Signaling through IL-2 receptor (IL-2R), a heterotrimeric complex sharing the gamma chain with IL-4 and IL-7

Disruption of IL-2–like genes in mice leads to colitis-like disease, indicating its essential role in immune response regulation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without authorization.

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