Thermo Fisher Scientific IL-17A Monoclonal Antibody (eBio17B7), Brilliant Violet 421, eBioscience

Thermo Fisher Scientific IL-17A Monoclonal Antibody (eBio17B7), Brilliant Violet 421, eBioscience

Applications and Tested Dilution

• Application: Flow Cytometry (Intracellular staining)
• Tested Dilution: ≤ 0.25 µg/test

Product Specifications

Available Formats

• Alexa Fluor 488
• APC
• Biotin
• Brilliant UV 395 / 615 / 661 / 737 / 805
• Brilliant Violet 650 / 711 / 786
• eFluor 450 / 506 / 660
• FITC
• PE / PE-Cyanine7 / PE-eFluor 610
• PerCP-Cyanine5.5
• Request custom conjugation available

Product Specific Information

The eBio17B7 antibody reacts with mouse and rat IL-17A without recognizing IL-17F. IL-17A is a CD4+ T cell-derived cytokine that promotes inflammatory responses and is elevated in autoimmune and inflammatory diseases such as rheumatoid arthritis, asthma, multiple sclerosis, psoriasis, and transplant rejection.

IL-23-dependent, IL-17A-producing CD4+ T cells (Th17 cells) are a distinct subset of helper T cells regulated negatively by Th1 and Th2 cytokines (IFN-γ, IL-4).

Applications Reported: Suitable for intracellular staining followed by flow cytometric analysis.
Applications Tested: Validated using mouse splenocytes and Intracellular Fixation & Permeabilization Buffer Set (Product #88-8824-00). Recommended ≤ 0.25 µg/test (100 µL final volume, 10⁵–10⁸ cells/test). Titrate for optimal performance.

Fluorochrome Information:
Brilliant Violet™ 421 emits at 423 nm; excitation at 407 nm (violet laser). Ensure cytometer compatibility.

Buffer Recommendation:
When using multiple polymer dye-conjugated antibodies, use Super Bright Complete Staining Buffer (Product #SB-4401-42) or Brilliant Stain Buffer™ (Product #00-4409-75) to minimize non-specific interactions.

BRILLIANT VIOLET™ is a trademark of Becton, Dickinson and Company, used under license. Powered by Sirigen™.

Target Information

Interleukin-17A (IL-17A, CTLA-8) is a 32 kDa disulfide-linked homodimer cytokine involved in inflammatory responses. It signals through the IL-17 receptor (IL-17R/CDw217), stimulating fibroblasts, epithelial, and endothelial cells to secrete IL-6, IL-8, G-CSF, and PGE2. IL-17A also enhances ICAM-1 expression and supports hematopoietic progenitor proliferation and neutrophil maturation. It regulates NF-κB and MAP kinase pathways, promoting COX-2 and nitric oxide production.

For Research Use Only. Not for diagnostic procedures or resale without authorization.

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