Thermo Fisher Scientific p16INK4a-TAT Polyclonal Antibody, PeproTech

Thermo Fisher Scientific p16INK4a-TAT Polyclonal Antibody, PeproTech

Applications and Tested Dilutions

• Western Blot (WB): 0.1–0.2 µg/mL
• ELISA: 0.5–2.0 µg/mL

Product Specifications

Product Specific Information

AA Sequence of recombinant protein:
EPAAGSSMEP SADWLATAAA RGRVEEVRAL LEAGALPNAP NSYGRRPIQV MMMGSARVAE LLLLHGAEPN CADPATLTRP VHDAAREGFL DTLVVLHRAG ARLDVRDAWG RLPVDLAEEL GHRDVARYLR AAAGGTRGSN HARIDAAEGP SDIPDGYGR KKRRQRRR

Preparation:
Produced from sera of rabbits immunized with highly pure Recombinant Human p16-INK4a-TAT. Anti-Human p16-INK4a-TAT-specific antibody was purified by affinity chromatography employing an immobilized Human p16-INK4a-TAT matrix.

Sandwich ELISA:
To detect Human p16-INK4a-TAT by sandwich ELISA (using 100 µL/well antibody solution), a concentration of 0.5–2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Human p16-INK4a-TAT (500-P284TBt) as a detection antibody, allows detection of at least 0.2–0.4 ng/well of Recombinant Human p16-INK4a-TAT.

Western Blot:
To detect Human p16-INK4a-TAT by Western Blot analysis, this antibody can be used at 0.1–0.2 µg/mL. When used with compatible secondary reagents, the detection limit for Recombinant Human p16-INK4a-TAT is 1.5–3.0 ng/lane under either reducing or non-reducing conditions.

Target Information

p16-INK4a is a nuclear protein that regulates the cell cycle by inhibiting cyclin-dependent kinase-4 (CDK4) and CDK6. It prevents CDK-cyclin D interaction, suppressing tumor formation and inducing replicative senescence in normal cells, including stem cells. Expression increases with age and accumulates in stem cell compartments. Mutations or deletions of the p16-INK4a gene are common in melanomas and other cancers. Protein transduction using TAT fusion proteins provides an alternative method for introducing transcription factors and nuclear proteins into cells.

For Research Use Only.
Not for use in diagnostic procedures or resale without authorization.

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