Thermo Fisher Scientific CD3 Monoclonal Antibody (UCHT1), Brilliant Violet 786, eBioscience
인간 CD3e를 인식하는 UCHT1 단클론 항체로, Brilliant Violet 786 형광체가 결합되어 있습니다. 유세포 분석용으로 검증되었으며, 405 nm 레이저 기반 장비에 적합합니다. 광민감성이 있으므로 암조건에서 보관해야 합니다. 연구용으로만 사용 가능합니다.
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Thermo Fisher Scientific CD3 Monoclonal Antibody (UCHT1), Brilliant Violet 786, eBioscience
Applications and Tested Dilution
- Application: Flow Cytometry
- Tested Dilution: 5 µL (0.25 µg)/test
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host/Isotype | Mouse / IgG1, kappa |
| Recommended Isotype Control | Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), Brilliant Violet™ 786, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | UCHT1 |
| Conjugate | Brilliant Violet™ 786 |
| Excitation/Emission Max | 406/788 nm |
| Form | Liquid |
| Concentration | 5 µL/test |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2925715 |
Additional Formats Available
- Unconjugated
- Alexa Fluor 532, 561, 660, 700
- APC, APC-eFluor 780
- Biotin
- Brilliant UV 395–805
- Brilliant Violet 421–711
- eFluor 450, 506
- FITC
- Functional Grade
- NovaFluor Blue, Red, Violet, Yellow series
- PE, PE-Cyanine5, PE-Cyanine7, PE-eFluor 610
- Super Bright 436–780
- Custom conjugation available
Product Specific Information
The UCHT1 monoclonal antibody reacts with human CD3e, a 20 kDa subunit of the TCR complex. The epsilon chain is required for proper assembly and surface expression of the TCR complex. CD3 is expressed by thymocytes and mature T cells.
- Reported Application: Flow cytometry
- Tested Application: Pre-diluted and validated for flow cytometric analysis of human peripheral blood cells
- Recommended Use: 5 µL (0.25 µg) per test in 100 µL final volume (10⁵–10⁸ cells/test)
Brilliant Violet™ 786 (BV786) emits at 786 nm and is compatible with violet (405 nm) laser-equipped cytometers. Use Super Bright Complete Staining Buffer (SB-4401-42) or Brilliant Stain Buffer™ (00-4409-75) to minimize non-specific interactions when using multiple polymer dyes.
- Light Sensitivity: Protect from light
- Fixation Compatibility: Compatible with IC Fixation Buffer (00-8222-49) or 1-step Fix/Lyse Solution (00-5333-54) for up to 3 days at 4°C. Not compatible with methanol-based fixation.
- Excitation/Emission: 407 nm / 786 nm
- Laser: Violet Laser
BRILLIANT VIOLET™ is a trademark of Becton, Dickinson and Company or its affiliates, used under license. Powered by Sirigen™.
Target Information
CD3 is a critical component of the T-cell receptor (TCR) complex, mediating antigen recognition and signal transduction. It includes subunits CD3 gamma, delta, epsilon, and zeta, encoded on human chromosome 11. CD3 is expressed on 68–82% of peripheral lymphocytes and 65–85% of thymocytes, but not on B or NK cells. Defects in CD3 genes are associated with CD3 immunodeficiency.
For Research Use Only. Not for use in diagnostic procedures.
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