Thermo Fisher Scientific CD3 Monoclonal Antibody (UCHT1), Brilliant Violet 786, eBioscience

Thermo Fisher Scientific CD3 Monoclonal Antibody (UCHT1), Brilliant Violet 786, eBioscience

Applications and Tested Dilution

• Application: Flow Cytometry
• Tested Dilution: 5 µL (0.25 µg)/test

Product Specifications

Additional Formats Available

• Unconjugated
• Alexa Fluor 532, 561, 660, 700
• APC, APC-eFluor 780
• Biotin
• Brilliant UV 395–805
• Brilliant Violet 421–711
• eFluor 450, 506
• FITC
• Functional Grade
• NovaFluor Blue, Red, Violet, Yellow series
• PE, PE-Cyanine5, PE-Cyanine7, PE-eFluor 610
• Super Bright 436–780
• Custom conjugation available

Product Specific Information

The UCHT1 monoclonal antibody reacts with human CD3e, a 20 kDa subunit of the TCR complex. The epsilon chain is required for proper assembly and surface expression of the TCR complex. CD3 is expressed by thymocytes and mature T cells.

• Reported Application: Flow cytometry
• Tested Application: Pre-diluted and validated for flow cytometric analysis of human peripheral blood cells
• Recommended Use: 5 µL (0.25 µg) per test in 100 µL final volume (10⁵–10⁸ cells/test)

Brilliant Violet™ 786 (BV786) emits at 786 nm and is compatible with violet (405 nm) laser-equipped cytometers. Use Super Bright Complete Staining Buffer (SB-4401-42) or Brilliant Stain Buffer™ (00-4409-75) to minimize non-specific interactions when using multiple polymer dyes.

• Light Sensitivity: Protect from light
• Fixation Compatibility: Compatible with IC Fixation Buffer (00-8222-49) or 1-step Fix/Lyse Solution (00-5333-54) for up to 3 days at 4°C. Not compatible with methanol-based fixation.
• Excitation/Emission: 407 nm / 786 nm
• Laser: Violet Laser

BRILLIANT VIOLET™ is a trademark of Becton, Dickinson and Company or its affiliates, used under license. Powered by Sirigen™.

Target Information

CD3 is a critical component of the T-cell receptor (TCR) complex, mediating antigen recognition and signal transduction. It includes subunits CD3 gamma, delta, epsilon, and zeta, encoded on human chromosome 11. CD3 is expressed on 68–82% of peripheral lymphocytes and 65–85% of thymocytes, but not on B or NK cells. Defects in CD3 genes are associated with CD3 immunodeficiency.

For Research Use Only. Not for use in diagnostic procedures.

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