Thermo Fisher Scientific CD4 Monoclonal Antibody (RM4-5), Brilliant Violet 786, eBioscience

Thermo Fisher Scientific CD4 Monoclonal Antibody (RM4-5), Brilliant Violet 786, eBioscience

Applications

• Flow Cytometry (Flow)

Tested Dilution: 1.0 µg/test

Product Specifications

Additional Formats

• Unconjugated
• Alexa Fluor 532, 561, 647, 660, 700
• APC, APC-eFluor 780, Biotin
• Brilliant UV 395–805, Brilliant Violet 421–711
• eFluor 450–615, FITC
• NovaFluor Blue/Yellow series
• Pacific Orange, PE, PE-Cyanine series, PerCP series
• Super Bright 436–780
• Custom conjugation available

Product Specific Information

The RM4-5 monoclonal antibody reacts with the mouse CD4 molecule, a 55 kDa cell surface receptor expressed by thymocytes, mature T cells, and dendritic cells. CD4 binds to MHC class II on antigen-presenting cells and plays a key role in T cell development and function. RM4-5 binding is blocked by GK1.5.

Applications Reported: Flow cytometric analysis
Applications Tested: Tested on mouse splenocytes at ≤1.0 µg/test (100 µL final volume). Optimal titration recommended.

Brilliant Violet™ 786 (BV786):

• Tandem dye emitting at 786 nm
• Requires violet (405 nm) laser
• Sensitive to light; protect from photo-oxidation

Staining Buffer Recommendation:
Use Super Bright Complete Staining Buffer (SB-4401) or Brilliant Stain Buffer™ (00-4409-75) when combining multiple polymer dyes to minimize non-specific interactions.

Fixation Compatibility:
Samples can be stored in IC Fixation Buffer (00-8222) or Fix/Lyse Solution (00-5333) for up to 3 days at 4°C in the dark. BV786 is not compatible with methanol-based fixation.

Excitation/Emission/Laser: 407 nm / 786 nm / Violet Laser

BRILLIANT VIOLET™ is a trademark or registered trademark of Becton, Dickinson and Company, used under license. Powered by Sirigen™.

Target Information

The CD4 antigen recognizes MHC class II molecules and serves as a co-receptor for HIV. Expressed mainly in T helper cells, as well as monocytes, macrophages, and dendritic cells. CD4 expression is found in thymus, lymph nodes, tonsils, spleen, and various non-lymphoid tissues. It assists in T-cell activation via association with Lck kinase and may play a role in neuronal damage in immune-related CNS diseases.

For Research Use Only. Not for diagnostic use or resale without authorization.

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