Thermo Fisher Scientific Ly-6G Monoclonal Antibody (1A8-Ly6g), Brilliant Violet 650, eBioscience
마우스 Ly-6G(Gr-1)에 특이적인 단클론 항체로, 중성구 표지 및 분석에 사용됩니다. Brilliant Violet 650 형광체 결합으로 유세포 분석에 최적화되어 있습니다. 1A8-Ly6g 클론은 중성구 선택적 결합 특성을 가지며, 비특이적 결합 최소화를 위해 Brilliant Stain Buffer 사용을 권장합니다.
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2개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific Ly-6G Monoclonal Antibody (1A8-Ly6g), Brilliant Violet 650, eBioscience
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Flow Cytometry (Flow) | 1.0 µg/test |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Host / Isotype | Rat / IgG2a, kappa |
| Recommended Isotype Control | Rat IgG2a kappa Isotype Control (eBR2a), Brilliant Violet™ 650, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 1A8-Ly6g |
| Conjugate | Brilliant Violet™ 650 |
| Excitation/Emission Max | 407 / 646 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage buffer | PBS, pH 7.2, with BSA |
| Contains | 0.09% sodium azide |
| Storage conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
Available Conjugate Formats
- Alexa Fluor 700
- APC
- APC-eFluor 780
- Biotin
- Brilliant UV 395 / 563 / 661 / 737
- Brilliant Violet 421 / 480 / 786
- eFluor 450
- FITC
- Functional Grade
- PE, PE-Cyanine5, PE-Cyanine7, PE-eFluor 610
- PerCP-eFluor 710
- Super Bright 436 / 600 / 702
- Request custom conjugation
Product Specific Information
Description:
The monoclonal antibody 1A8-Ly6g reacts with mouse Ly-6G (Gr-1), a 25-kDa GPI protein expressed exclusively by neutrophils. It plays a role in neutrophil infiltration, recruitment, and migration, and associates with B2 integrins CD11a and CD11b. The ligand for Ly-6G remains unknown.
Unlike the RB6-8C5 antibody, 1A8-Ly6g binds only to Ly-6G on neutrophils and has been used for neutrophil depletion with reported antagonist activity.
Applications Reported:
Flow cytometric analysis.
Applications Tested:
Tested by flow cytometric analysis of mouse bone marrow cells. Recommended ≤1.0 µg per test (100 µL final volume). Optimize antibody titration empirically for best results.
Fluorochrome Information:
Brilliant Violet™ 650 (BV650) emits at 649 nm and is suitable for cytometers equipped with a violet (405 nm) laser. Ensure instrument compatibility before use.
Buffer Recommendations:
When using multiple polymer dye-conjugated antibodies, use Super Bright Complete Staining Buffer (Cat. # SB-4401-42) or Brilliant Stain Buffer™ (Cat. # 00-4409-75) to minimize non-specific interactions.
Light Sensitivity:
Protect from light to prevent photo-induced oxidation.
Fixation:
Samples can be fixed with IC Fixation Buffer (Cat. # 00-8222-49) or 1-step Fix/Lyse Solution (Cat. # 00-5333-54) and stored up to 3 days at 4°C in the dark. BV650 is not compatible with methanol-based fixation.
Excitation: 407 nm
Emission: 649 nm
Laser: Violet Laser
BRILLIANT VIOLET™ is a trademark or registered trademark of Becton, Dickinson and Company or its affiliates, and is used under license. Powered by Sirigen.
Target Information
Ly6G (Lymphocyte antigen 6 complex locus G6D):
A component of the myeloid differentiation antigen Gr-1, expressed by myeloid-derived cells in bone marrow (21–25 kDa). Ly6G is a marker for neutrophils, monocytes, and granulocytes. Expression correlates with granulocyte differentiation and maturation. It may play a role in anti-tumor responses and modulation of local immune activity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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