Thermo Fisher Scientific CD20 Chimeric Recombinant Rabbit Monoclonal Antibody (18B12)
CD20 단백질을 인식하는 키메릭 재조합 토끼 모노클로날 항체로, 마우스 CD20에 특이적입니다. Western blot, ICC/IF, Flow cytometry 등 다양한 응용에 적합하며, HEK293 세포 발현 시스템 기반으로 생산되었습니다. Protein A로 정제된 고순도 액상 항체입니다.
- 판매단위
- pk
카탈로그
1개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific CD20 Chimeric Recombinant Rabbit Monoclonal Antibody (18B12)
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | Assay-dependent |
| Immunocytochemistry (ICC/IF) | 10 µg/mL |
| Flow Cytometry (Flow) | 1:100 |
| Functional Assay | Assay-dependent |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Mouse |
| Host / Isotype | Rabbit / IgG, kappa |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | 18B12 |
| Immunogen | Generated by immunizing CD20-/- mice with CD20-transfected cell lines (300.18 and 70Z3) and CD20-peptide-keyhole limpet hemocyanin conjugate |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS |
| Contains | 0.02% ProClin 300 |
| Storage Conditions | Store at 4°C short term; for long term storage, store at -20°C avoiding freeze/thaw cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_3074619 |
Product Specific Information
Specificity:
This antibody is specific for murine CD20, a 33–37 kDa non-glycosylated surface phosphoprotein expressed on mature and most malignant B cells, but not on stem cells or plasma cells.
Target Information
CD20 is a non-glycosylated surface phosphoprotein (33–37 kDa depending on phosphorylation) expressed on mature and most malignant B cells, a subpopulation of T lymphocytes, and follicular dendritic cells.
It regulates transmembrane calcium conductance, cell cycle progression, and B-cell proliferation. CD20 associates with lipid rafts, with intensity depending on extracellular triggers and BCR aggregation.
After receptor ligation, BCR and CD20 colocalize and rapidly dissociate before BCR endocytosis, while CD20 remains on the cell surface.
CD20 is a key target for antibody-mediated B-cell depletion therapies, as it is highly expressed on most B-cell malignancies and remains membrane-bound following treatment.
Diseases associated with CD20 dysfunction include Ms4a1-related common variable immune deficiency.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Thermo Fisher Scientific 상품 둘러보기
전체보기
문의
0개 · 배송·재고 문의는 실시간 상담이 빠릅니다아직 등록된 문의가 없어요.